Biological activities of essential oils from six genotypes of four Ocotea species

Michele Andréia Rambo; Krissie Daian Soares; Letícia Jacobi Danielli; Daiane Flores  Dalla Lana; Sérgio Augosto de Loreto Bordignon; Alexandre Meneghello Fuentefria; Miriam Apel

doi:10.1590/s2175-97902022e181097

Authors

Michele Andréia Rambo Pharmaceutical Sciences Graduate Program, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
Krissie Daian Soares Pharmaceutical Sciences Graduate Program https://orcid.org/0000-0001-5195-1269
Letícia Jacobi Danielli Pharmaceutical Sciences Graduate Program, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
Daiane Flores Dalla Lana Pharmaceutical Sciences Graduate Program, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
Sérgio Augosto de Loreto Bordignon Environmental Impact Assessment Graduate Program, La Salle University Center, Canoas, Rio Grande do Sul, Brazil
Alexandre Meneghello Fuentefria Pharmaceutical Sciences Graduate Program, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil
Miriam Apel Pharmaceutical Sciences Graduate Program

DOI:

https://doi.org/10.1590/s2175-97902022e181097

Keywords:

Antifungal, Biological activity, Essential oil, Lauraceae, Ocotea

Abstract

Essential oils from four Ocotea species collected in southern Brazil were evaluated for chemical composition using gas chromatography coupled with mass spectrometry. The primary compound identified in O. acutifolia essential oil was an unsaturated tetracyclic diterpene, phyllocladene (67.7%), followed by a sesquiterpene hydrocarbon, β-selinene (18.0%). The sesquiterpene fraction was predominant in oils from two collections of O. puberula; β-caryophyllene (25.2%) and globulol (22.6%) were the major compounds identified in collections 1 and 2, respectively.

O. silvestris essential oil contained predominantly germacrene D and bicyclogermacrene. These compounds were also predominant in essential oil from O. indecora leaves collected from shady habitats. By contrast, essential oil extracted from O. indecora grown under direct sunlight contained mainly oxygenated sesquiterpenes, such as guaiol (30.2%), α-eudesmol (27.6%), and β-eudesmol (12.7%). Chemotaxis assays showed that Ocotea essential oils had no significant inhibitory activity on leukocyte migration compared with a chemotactic stimulant (lipopolysaccharide from Escherichia coli). However, the oils exhibited antifungal activity against Candida parapsilosis, with a minimum inhibitory concentration of 500 µg/mL. To our knowledge, this is the first study to investigate the in vitro antifungal and antichemotactic activities of essential oils from Ocotea species native to southern Brazil.

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Biological activities of essential oils from six genotypes of four Ocotea species

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