Investigation of therapeutic effects in the wound healing of chitosan/pGM-CSF complexes
DOI:
https://doi.org/10.1590/s2175-97902022e19668Keywords:
Wound healing, Chitosan, pGM-CSF, ComplexesAbstract
Granulocyte macrophage colony-stimulating factor (GM-CSF) has been shown to promote the growth, proliferation, and migration of endothelial and keratinocyte cells. Chitosan has been widely used as a biopolymer in wound-healing studies. The aim of this study was to investigate the in vitro proliferative effects of chitosan/pGM-CSF complexes as well as the therapeutic role of the complexes in an in vivo rat wound model. The effect of complexes on cell proliferation and migration was examined. Wounds were made in Wistar-albino rats, and examined histopathologically. The cell proliferation and migration were increased weight ratio- and time-dependently in HaCaT and NIH-3T3 cell lines. Wound healing was significantly accelerated in rats treated with the complexes. These results showed that the delivery of pGM-CSF using chitosan complexes could play an accelerating role in the cell proliferation, migration, and wound-healing process.
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Agirre M, Zarate J, Ojeda E, Puras G, Desbrieres J, Pedraz JL. Low molecular weight chitosan (lmwc)-based polyplexes for pdna delivery: from bench to bedside. Polymers. 2014;6(6):1727-1755.
Ampuero FO, Vidal A, Concha M, Morales J, Orellana S, Villoslada IM. Nanoparticles for the treatment of wounds. Curr Pharm Des. 2015;21(29):4329-4341.
Barrientos S, Brem H, Stojadinovic O, Tomic-Canic M. Clinical application of growth factors and cytokines in wound healing. Wound Rep Regen. 2014;22(5):569-578.
Boateng JS, Matthews KH, Stevens HNE, Eccleston GM. Wound healing dressings and drug delivery systems: A review. J Pharm Sci. 2008;97(8):2892-2923.
Bryan D, Walker KB, Ferguson M, Thorpe R. Cytokine gene expression in a murine wound healing model. Cytokine. 2005;31(6):429-438.
Chang J, Liu W, Han B, Peng S, Bin H, Gu Z. Investigation of the skin repair and healing mechanism of N-carboxymethyl chitosan in second-degree burn wounds. Wound Repair Regen. 2013;21(1):113-121.
Değim Z. Use of microparticulate systems to accelerate skin wound healing. J Drug Targeting. 2008;16(6):437-448.
Doan CC, Le LT, Hoang SN, Do SM, Le DV. Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells. Biol Res. 2014;47(70):1-15
Duceppe N, Tabrizian M. Factors influencing the transfection efficiency of ultra low molecular weight chitosan/hyaluronic acid comparticles. Biomaterials. 2009;30(13):2625-2631.
Eming SA, Krieg T, Davidson JM. Gene therapy and wound healing. Clin Dermatol. 2007;25(1):79-92.
Enoc S. Basic science of wound healing. Surgery. 2007;26(2):31-37.
Eriksson E, Vranckx J. Wet wound healing: from laboratory to patients to gene therapy. Am J Surg. 2004;188(1):36-41.
Gauglitz GG, Jeschke MG. Combined gene and stem cell therapy for cutaneous wound healing. Mol Pharm. 2011;8(5):1471-1479.
Greaves NS, Iqbal SA, Baguneid M, Bayat A. The role of skin substitutes in the management of chronic cutaneous wounds. Wound Repair Regen . 2013;21(2):194-210.
Grimstad Ø, Sandanger Ø, Bioengineer LR, Otterdal K, Damaas JK, Pukstad B, et al. Cellular sources and inducers of cytokines present in acute wound fluid. Wound Rep Regen . 2011;19(3):337-347.
Heo SH, Han KB, Lee YJ, Kim JH, Yoon KH, Han MD, et al. Recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) could accelerate burn wound healing in hamster skin. Animal Cells Syst. 2012;16(3):207-214.
Kirchner LM, Meerbaum SO, Gruber BS, Knoll AK, Bulgrin J, Taylor RA, et al. Effects of vascular endothelial growth factor on wound closure rates in the genetically diabetic mouse model. Wound Repair Reg. 2003;11(2):127-131.
Köping-Höggård M, Varum KM, Issa M, Danielsen S, Christensen BE, Stokke BT, et al. Improved chitosan-mediated gene delivery based on easily dissociated chitosan polyplexes of highly defined chitosan oligomers. Gene Ther. 2004;11(19):1441-1452.
Lasa CI, Kidd RR, Nunez HA, Drohan WN. Effect of fibrin glue and opsite on open wounds in DB/DB mice. J Surgical Res 1993;54(3):202-206.
Lavertu M, Methot S, Tran-Khanh N, Buschmann MD. High efficiency gene transfer using chitosan/DNA comparticles with specific combinations of molecular weight and degree of deacetylation. Biomaterials. 2006;27(27):4815-4824.
Lee M, Nah JW, Kwon Y, Koh JJ, Ko KS, Kim SW. Water-soluble and low molecular weight chitosan-based plasmid DNA delivery. Pharm Res. 2001;18(4):427-431.
Li J, Chen J, Kirsner R. Pathophysiology of acute wound healing. Clin Dermatol . 2007;25(1):9-18.
Li X, Nan K, Li L, Zhang Z, Chen H. In vivo evaluation of curcumin nanoformulation loaded methoxy poly(ethylene glycol)-graft-chitosan composite film for wound healing application. Carbohydr Polym. 2012;88(1):84-90.
Mann A, Breuhahn K, Schirmacher P, Blessing M. Keratinocyte-derived granulocyte-macrophage colony stimulating factor accelerates wound healing: stimulation of keratinocyte proliferation, granulation tissue formation, and vascularization. J Invest Dermatol. 2001;117(6):1382-1390.
Miguel SP, Moreira AF, Correia IJ. Chitosan based-asymmetric membranes for wound healing: A review. Int J Biol Macromol. 2019;127:460-475.
Minagawa T, Okamura Y, Shigemasa Y, Minamia S, Okamoto Y. Effects of molecular weight and deacetylation degree of chitin/chitosan on wound healing. Carbohydr Polym . 2007;67(4):640-644.
Patrulea V, Ostafe V, Borchard G, Jordan O. Chitosan as a starting material for wound healing applications. Eur J Pharm Biopharm. 2015;97(Pt B):417-426.
Paudel KS, Milewski M, Swadley CL, Brogden NK, Priyanka G, Stinchcomb AL. Challenges and opportunities in dermal/transdermal delivery. Ther Deliv. 2010;1(1):109-131.
Ribeiro MP, Espiga A, Silva D, Baptista P, Henriques J, Ferreira C, et al. Development of a new chitosan hydrogel for wound dressing. Wound Repair Regen . 2009;17(6):817-824.
Riedel K, Riedel F, Goessler UR, Holle G, Germann G, Sauerbier M. Current status of genetic modulation of growyh factors in wound repair. Int J Mol Med. 2006;17(2):183-193.
Salva E, Turan SO, Akbuğa J. The increased of in vitro cell proliferation by using chitosan/pGM-CSF complexes. Ind J Pharm Sci . 2011;73(2):131-138.
Samadikuchaksaraei A, Nasiri N, Sharifi MJ, Chauhan NPS, Farhadihosseinabadi B, Sovini SF, et al. Intravenous administration of granulocyte-colony stimulating factor for stem cells mobilization and third degree burn wound healing in rats. J Appl Biotechnol Rep. 2019;6(3):83-87.
Singh R, Shitiz K, Singh A. Chitin and chitosan: biopolymers for wound management. Int Wound J. 2017;14(6):1276-1289.
Tsao CT, Chang CH, Lin Y, Wu MF, Wang JL, Young TH, et al. Evaluation of chitosan/γ-poly(glutamic acid) polyelectrolyte complex for wound dressing materials. Carbohydr Polym . 2011;84(2):812-819.
Turan S, Akbuğa J. Plasmid DNA-loaded chitosan/TPP comparticles for topical gene delivery. Drug Del. 2011;18(3): 215-222
Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol Rev. 2003;83(3):836-870.
Williamson D, Harding K. Wound healing. Medicine. 2004;32(12):4-7.
Wu P, Chen H, Jin R, Weng T, Ho JK, You C, et al. Non-viral gene delivery systems for tissue repair and regeneration. J Transl Med. 2018;16(1):29.
Yan D, Liu S, Zhao X, Bian H, Yao X, Xing J, et al. Recombinant human granulocyte macrophage colony stimulating factor in deep second-degree burn wound healing. Medicine. 2017;96(22):(e6881).
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Türkiye Bilimsel ve Teknolojik Araştırma Kurumu
Grant numbers SBAG-HD-155, 106S232
