Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds

Authors

  • João Paulo Campos Andrade Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Osvaine Junior Alvarenga Alves Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Marian Caroline Costa Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Valéria Maria Melleiro Gimenez Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Iara Silva Squarisi Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Heloiza Diniz Nicolella Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Mariana Cintra Pagotti Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Denise Crispim Tavares Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Wilson Roberto Cunha Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Márcio Luís Andrade e Silva 1 Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil https://orcid.org/0000-0002-9442-4757
  • Ana Helena Januario 1 Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Lizandra Guidi Magalhães Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil
  • Patrícia Mendonça Pauletti Center for Research in Exact and Technological Sciences, University of Franca, Franca, São Paulo, Brazil https://orcid.org/0000-0003-1831-0050

DOI:

https://doi.org/10.1590/s2175-97902022e20278%20

Keywords:

Cytotoxicity, Flavonol, HPLC-HRMS-DAD, Iridoid, Leishmanicidal

Abstract

Adenocalymma axillarum (K.Schum.) L.G. Lohmann is a liana belonging to the family Bignoniaceae. In traditional medicine, the genus Adenocalymma is used to treat fever, skin ailments, and body, joint, and facial muscle pains, and it is also applied as cosmetic. Biological assays conducted with the A. axillarum crude leaf ethanol extract have indicated leishmanicidal activity and absence of cytotoxicity. This study aimed to analyze the A. axillarum leaf ethanol crude extract by high-performance liquid chromatography-high-resolution mass spectrometry- diode array detector (HPLC-HRMS-DAD) and to evaluate the leishmanicidal and cytotoxic activities of this crude extract, its fractions, and isolated compounds. HPLC-HRMS-DAD analysis of this extract revealed that it consisted mainly of flavonoids, with nine major compounds. Extract purification yielded 4-hydroxy-N-methylproline, 6-β-hydroxyipolamiide, quercetin-3-O-robinobioside, hyperin, isorhamnetin-3-O-robinobioside, and 3’-O-methylhyperin, which were identified by Nuclear Magnetic Resonance. The isolated compounds were inactive against Leishmania amazonensis promastigotes and human lung fibroblast cells.

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Published

2022-11-25

Issue

Vol. 58 (2022)

Section

Original Article

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How to Cite

Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds. (2022). Brazilian Journal of Pharmaceutical Sciences, 58. https://doi.org/10.1590/s2175-97902022e20278