HPLC-DAD analysis, antifungal and antioxidant activity of Solanum dolichosepalum bitter extracts and fractions
DOI:
https://doi.org/10.1590/s2175-97902022e20350Keywords:
Antifungal Activity, Antioxidant Activity, HPLC-DAD Analysis, Solanum Dolichosepalum, Candida Albicans, Fusarium OxysporumAbstract
Solanum dolichosepalum is a plant with anti-infective effects. It is a healing agent and has ethnopharmacological uses. In this study, the antifungal activity of extracts and fractions of this species on C. albicans and F. oxysporum was evaluated. The antioxidant activity was measured using the ABTS and DPPH methods, and by determining the total content of phenolic compounds. An HPLC-DAD qualitative analysis was carried out to identify phenolic compounds and alkaloids. Pearson’s correlation coefficients were calculated. Inhibitory effects were found in all the extracts and fractions on the analyzed microorganisms. F. oxysporum was the microorganism most sensitive to the action of S. dolichosepalum extracts. All extracts and fractions showed antioxidant activity, with the acetone extract and the acetone fraction being those that generated the best results. The content of total phenolic compounds showed that acetone has a greater affinity with the phenolic compounds present in S. dolichosepalum. In this plant, p-Hydroxybenzoic, vanillic, ferulic, trans-cinnamic, caffeic, p-coumaric, and rosmarinic acids were found, as well as theobromine, quercetin, and luteolin. The content of total phenolic compounds was determined to be directly proportional to the inhibition of the ABTS and DPPH radicals, and the inhibition of the analyzed microorganisms. It was determined that the extracts and fractions obtained from S. dolichosepalum show antioxidant and antifungal activity
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Alothman M, Bhat R. Karim, A. Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food Chem. 2009;115(3):785-788.
Alves C, Ferreira I, Barros L, Silva S, Azeredo J, Henriques M. Antifungal activity of phenolic compounds identified in flowers from North Eastern Portugal against Candida species. Future Microbiol. 2014;9(2):139-146.
Anaya-López J, Lopez-Meza J, Baizabal-Aguirre V, Cano- Camacho H, Ochoa-Zarzosa A. Fungicidal and cytotoxic activity of a Capsicum chinense defensin expressed by endothelial cells. Biotechnol Lett. 2006;28(14):1101-1108. doi: 10.1007/s10529-006-9060-4
» https://doi.org/10.1007/s10529-006-9060-4
Andre C, Oufir M, Guignard C, Hoffmann L, Hausman J, Evers D, et al. Antioxidant profiling of native Andean potato tubers (Solanum tuberosum L.) reveals cultivars with high levels of β-carotene, α-tocopherol, chlorogenic acid, and petanin. J Agric Food Chem. 2007;55(26):10839-10849.
Arango M, Bueno J, Isaza G, Pérez J, Álvarez L, Osorio E, et al. Efectos antibacterianos y antimicóticos de Alternanthera williamsii, Solanum dolichosepalum, Baccharis trinervis, Tabebuia chrysantha y Phenax rugosus Biosalud. 2004;3:49-54.
Baño M, Lorente J, Castillo J, Benavente O, del Río J, Ortuño A.,et al. Phenolic Diterpenes, Flavones, and Rosmarinic Acid Distribution during the Development of Leaves, Flowers, Stems, and Roots of Rosmarinus officinalis Antioxidant Activity. J Agric Food Chem . 2003;51(15):4247-4253. doi: 10.1021/jf0300745.
» https://doi.org/10.1021/jf0300745
Brown C. Antioxidants in potato. Am J Potato Res. 2005;82(2):163-172.
Choi S, Kim H, Kim H, Lee I, Kozukue N, Levin C, Friedman M. Free Amino Acid and Phenolic Contents and Antioxidative and Cancer Cell-Inhibiting Activities of Extracts of 11 Greenhouse-Grown Tomato Varieties and 13 Tomato-Based Foods. J Agric Food Chem . 2011;59(24):12801-12814. doi: 10.1021/jf202791j
» https://doi.org/10.1021/jf202791j
Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol. 2012;23(2):174-181.
Das J, Lahan J, Srivastava R. Solanum melongena: A potential source of antifungal agent. Indian J Microbiol. 2010;50(1):62-69. doi: 10.1007/s12088-010-0004-2
» https://doi.org/10.1007/s12088-010-0004-2
De Bedout C, Ayabaca J, Vega R, Méndez M, Santiago Á, Pabón M,et al. Evaluación de la susceptibilidad de especies de Candida al fluconazol por el método de difusión de disco. Biomédica. 2003;23(1):1-37.
De Granada E, De Amézquita M, Bautista G, Valencia H. Fusarium oxysporum el hongo que nos falta conocer. Acta Biol Colomb. 2001;6(1):7.
Dragovic V, Levaj B, Mrkic V, Bursac D, Boras M. The content of polyphenols and carotenoids in three apricot cultivars depending on stage of maturity and geographical region. Food Chem. 2007;102(3):966-975. doi: http://dx.doi.org/10.1016/j.foodchem.2006.04.001
» https://doi.org/http://dx.doi.org/10.1016/j.foodchem.2006.04.001
Floegel A, Kim D, Chung S, Koo S, Chun O. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compos Anal. 2011;24(7):1043-1048. doi: http://dx.doi.org/10.1016/j.jfca.2011.01.008
» https://doi.org/http://dx.doi.org/10.1016/j.jfca.2011.01.008
Guzmán C, Durán Mendoza T, Silva Belmares S, Sierra Rivera C, Pérez Guzmán A. Polifenoles, taninos y actividad antioxidante de extracto de flor y hoja de Solanum marginatum Invest Desarrollo Cienc Tecnol Aliment. 2016;1(2):442-447.
Huang H, Syu K, Lin J. Chemical composition of Solanum nigrum linn extract and induction of autophagy by leaf water extract and its major flavonoids in AU565 breast cancer cells. J Agric Food Chem . 2010;58(15):8699-8708.
Ieri F, Innocenti M, Andrenelli L, Vecchio V, Mulinacci N. Rapid HPLC/DAD/MS method to determine phenolic acids, glycoalkaloids and anthocyanins in pigmented potatoes (Solanum tuberosum L.) and correlations with variety and geographical origin. Food Chem . 2011;125(2):750-759.
Lee J, Park J, Kim Y, Han Y. Chlorogenic acid, a polyphenolic compound, treats mice with septic arthritis caused by Candida albicans Int Immunopharmacol. 2008;8(12):1681-1685. doi: http://doi.org/10.1016/j.intimp.2008.08.002
» http://doi.org/10.1016/j.intimp.2008.08.002
Magalhães F, Cassiano D, Branco C, Silva T, Lins A, Silva T, et al. Antioxidant Activity and Phenolics Analysis by HPLC- DAD of Solanum thomasiifolium Sendtner (Solanaceae). Free Radicals Antioxid. 2014;4(1):15-23.
Marin Á, López C, Pérez J, Isaza G. Actividad antifungica de los extractos acuosos de Baccharis trinervis, Baccharis latifolia y Solanum dolichosepalum Biosalud . 2006;(5):51-59.
Martin D, Cárdenas, O, Pacheco J, Cárdenas C, Gómez J. Antifungal activity of chloroform and acetone extracts of Solanum dolichosepalum against Fusarium oxysporum Int J Pharm Pharm Sci. 2016;8(8):373-374.
N’Dri D, Calani L, Mazzeo T, Scazzina F, Rinaldi M, Del Rio D, et al. Effects of different maturity stages on antioxidant content of Ivorian Gnagnan (Solanum indicum L.) berries. Molecules. 2010;15(10):7125-7138.
Nossa González D, Talero Pérez Y, Rozo Núñez W. Determinación del contenido de polifenoles y actividad antioxidante de los extractos polares de comfrey (Symphytum officinale L). Rev Cubana Plant Med. 2016;21:125-132.
Ortíz C, Guerrero M, Pérez C, Martin D. Actividad antibacteriana de extractos alcohólicos de hojas de Solanum dolichosepalum (Bitter). Informador Técnico. 2019;83(2):121-130.
Perez J, Isaza G, Bueno J, Arango M, Nieto A, Londoño D. Efecto de los extractos de Phenax rugosus, Tabebuia chrysantha, Althernantera illiamsii y Solanum dolichosepalum sobre el leucograma y la producción de anticuerpos en ratas. Rev Méd Risaralda. 2004;10(2):13-21.
Potawale S, Sinha S, Shroff K, Dhalawat H, Boraste S, Gandhi S, et al. Solanum nigrum Linn: A Phytopharmacological Review. Pharmacology online. 2008;3:140-163.
Priyadharshini D, Sujatha V. Antioxidant profile and GC-MS analysis of Solanum erianthum leaves and stem. A comparison. Int J Pharm Pharm Sci . 2013;5(3):652-658.
Puertas-Mejía M, Gómez-Chabala L, Rojano B, Sáez-Veja J. Capacidad antioxidante in vitro de fracciones de hojas de Piper peltatum L. Rev Cubana Plant Med . 2009:14(2):0-0.
Quideau S, Deffieux D, Douat C, Pouységu L. Plant Polyphenols: Chemical Properties, Biological Activities, and Synthesis. Angew Chem Int Ed. 2011;50(3):586-621. doi: 10.1002/anie.201000044
» https://doi.org/10.1002/anie.201000044
Ramírez Cárdenas A, Isaza Mejía G, Pérez Cárdenas J. Vegetal Species Studied By Their Antimicrobial, Immunomodulatory And Hypoglicemic Properties In Caldas-Colombia, South America. Biosalud . 2013;12(1):59-82.
Ramírez Cárdenas A, Isaza Mejía G, Pérez Cárdenas J, Garzón M, Maby M. Estudio fitoquímico preliminar y evaluación de la actividad antibacteriana del Solanum dolichosepalum Bitter (Frutillo). Ver Cubana Plant Med. 2017;22(1).
Segundo M. Candida albicans, un hongo oportunista. 2006. Sección Salud (38). Retrieved 24-03, 2014, from Retrieved 24-03, 2014, from http://www.miherbolario.com/articulos/salud/6/candida-albicans-un-hongo-oportunista
» http://www.miherbolario.com/articulos/salud/6/candida-albicans-un-hongo-oportunista
Sticher O. Natural product isolation. Natural product reports. 2008;25(3):517-554.
Sulaiman S, Yusoff N, Eldeen I, Seow E, Sajak A, Ooi KL. Correlation between total phenolic and mineral contents with antioxidant activity of eight Malaysian bananas (Musa sp.). J Food Compos Anal . 2011;24(1):1-10. doi: http://dx.doi.org/10.1016/j.jfca.2010.04.005
» https://doi.org/http://dx.doi.org/10.1016/j.jfca.2010.04.005
Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Hawkin D. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal . 2006;19(6-7):669-675. doi: http://dx.doi.org/10.1016/j.jfca.2006.01.003
» http://dx.doi.org/10.1016/j.jfca.2006.01.003
Vasco C, Avila J, Ruales J, Svanberg U, Kamal-Eldin A. Physical and chemical characteristics of golden-yellow and purple-red varieties of tamarillfigo fruit (Solanum betaceum Cav.). Int J Food Sci Nutr. 2009;60(sup7):278-288.
Wang H, Chung P, Wu C, Lan K, Yang M, Wang C. Solanum nigrum L. polyphenolic extract inhibits hepatocarcinoma cell growth by inducing G2/M phase arrest and apoptosis. J Sci Food Agric. 2010;91(1):178-185.
Zadra M, Piana M, Brum T, Boligon A, Freitas R, Machado M. Antioxidant Activity and Phytochemical Composition of the Leaves of Solanum guaraniticum A. St.-Hil. Molecules . 2012;17(11):12560-12574.
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