Study of the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects

Authors

  • Jian feng Mei College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P. R. China https://orcid.org/0000-0002-4528-0232
  • Shao fen Cai College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P. R. China
  • Yu Yi College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P. R. China
  • Xu dong Wang College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P. R. China
  • Guo qing Ying College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, P. R. China https://orcid.org/0000-0001-5683-7561

DOI:

https://doi.org/10.1590/s2175-97902022e201004

Keywords:

Serrapeptase, Protease, Fibrinolytic activity, In vitro thrombolysis

Abstract

Serrapeptase, a proteolytic enzyme, has been used for the adjuvant treatment of many diseases. However, its fibrinolytic activity is still uncertain. Herein, the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects were investigated. The results showed that the fibrinolytic activity of serrapeptase was 1295 U/mg, and the specific activity was 3867 U/mg of protein when its proteolytic activity toward casein was 2800 U/mg. The optimum temperature and pH for serrapeptase activity were 37-40°C and 9.0, respectively. At 1 mmol/L, Zn2+, Mn2+ and Fe2+ could activate the fibrinolytic activity of serrapeptase, while K+, Cu2+, sodium dodecyl sulfate (SDS) and ethylene diamine tetraacetic acid (EDTA) inhibited it. In vitro tests showed that serrapeptase could completely prevent blood coagulation at 150 U/mL, and the percentage of blood clot lysis reached 96.6% at 37°C after 4 h at 300 U/mL. These results indicate that serrapeptase has excellent fibrinolytic activity, and can be used as a health food or candidate drug for the prevention or treatment of thrombotic diseases.

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ETHICS STATEMENT

This article does not contain any studies with human participants or animals performed by any of the authors.

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Published

2023-01-27

Issue

Vol. 58 (2022)

Section

Original Article

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How to Cite

Study of the fibrinolytic activity of serrapeptase and its in vitro thrombolytic effects. (2023). Brazilian Journal of Pharmaceutical Sciences, 58. https://doi.org/10.1590/s2175-97902022e201004