Anti-diabetic effects of fullerene C60 nanoparticle mediated by its anti-oxidant activity in the pancreas in type 1 diabetic rats
DOI:
https://doi.org/10.1590/s2175-97902022e201077Keywords:
Fullerene, Streptozotocine, Diabetes Mellitus, Oxidative Stress, Pancreas, RatAbstract
The present study aims to examine the anti-diabetic effects of fullerene C60 nanoparticle, as an anti-oxidant compound, on serum glucose level, body weight, food and water intake, and pancreatic oxidative stress in the rats with type 1 diabetes. Diabetes mellitus was induced by single intravenous injection of streptozotocine (45 mg/kg) into the tail vein of the rats. Four groups of rats were divided as follow: normal, normal treatment, diabetic, and diabetic treatment groups. Normal treatment and diabetic treatment groups received intra-orally fullerene (1 mg/ kg/daily) up to day 60 following streptozotocine injection. Oxidative stress markers in the pancreas were evaluated on day 60 after inducing diabetes mellitus. Injection of streptozotocine significantly increased serum glucose level as well as food and water intake on all experimental days; it decreased body weight on day 60. Streptozotocine increased MDA level and decreased GSH level and SOD activity in the pancreas. Fullerene significantly decreased food and water intake and increased body weight as compared with the diabetic group. Fullerene also could normalize the pancreatic MDA and GSH markers. The present study suggested that fullerene can decrease diabetic symptoms via its anti-oxidant activity in the pancreas in the rats with type 1 diabetes mellitus.
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Akhtar MJ, Ahamed M, Alhadlaq HA, Alshamsan A. Mechanism of ROS scavenging and antioxidant signalling by redox metallic and fullerene nanomaterials: Potential implications in ROS associated degenerative disorders. Biochim Biophys Acta Gen Subj. 2017;1861(4):802-813.
Askary-Ashtiani A, Ghanjal A, Motaqi M, Meftahi GH, Hatef B, Niknam H. The Isokinetic and Electromyographic Assessment of Knee Muscles Strength in the Short-and Long-Term Type 2 Diabetes. Asian J Sports Med. 2016;7(4):e37008.
Bal R, Türk G, Tuzcu M, Yilmaz O, Ozercan I, Kuloglu T, et al. Protective effects of nanostructures of hydrated C60 fullerene on reproductive function in Streptozotocin-diabetic male rats. Toxicology. 2010;282(3):69-81.
Barragán-Bonilla MI, Mendoza-Bello JM, Aguilera P, Parra-Rojas I, Illades-Aguiar B, Ramírez M, et al. Combined administration of streptozotocin and sucrose accelerates the appearance of type 2 diabetes symptoms in rats. J Diabet Res. 2019;2019:3791061.
Bayatpoor ME, Mirzaee S, Abd MK, Mohammadi MT, Shahyad S, Bahari Z, et al. Crocin treatment decreased pancreatic atrophy, LOX-1 and RAGE mRNA expression of pancreas tissue in cholesterol-fed and streptozotocin-induced diabetic rats. J Complement Integr Med. 2019;17(2):DOI: 10.1515/jcim-2019-0117.
» https://doi.org/10.1515/jcim-2019-0117.
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248-254.
Cepas V, Collino M, Mayo JC, Sainz RM. Redox signaling and advanced glycation endproducts (AGEs) in diet-related diseases. Antioxidants. 2020;9(2):142.
Chen YH, Chen ZW, Li HM, Yan XF, Feng B. AGE/RAGE-induced EMP release via the NOX-derived ROS pathway. J Diabet Res . 2018;2018:6823058.
Chistyakov VA, Smirnova YO, Prazdnova EV, Soldatov AV. Possible mechanisms of fullerene C60 antioxidant action. Biomed Res Int. 2013;2013:821498.
Ebaid H, Bashandy SA, Alhazza IM, Hassan I, Al-Tamimi J. Efficacy of a methanolic extract of adansonia digitata leaf in alleviating hyperglycemia, hyperlipidemia, and oxidative stress of diabetic rats. Biomed Res Int. 2019;2019:2835152.
Ebrahimi MJ, Aliaghaei A, Boroujeni ME, Khodagholi F, Meftahi G, Abdollahifar MA, et al. Human umbilical cord matrix stem cells reverse oxidative stress-induced cell death and ameliorate motor function and striatal atrophy in rat model of Huntington disease. Neurotox Res. 2018;34(2):273-284.
Florence NT, Théophile D, Désiré DD, Bertin V, Etienne D, Beauwens R, et al. Antidiabetic activities of methanol-derived extract of Dorstenia picta twigs in normal and streptozotocin-induced diabetic rats. Asian J Trad Med. 2007;2(4):140-148.
Galvan YP, Alperovich I, Zolotukhin P, Prazdnova E, Mazanko M, Belanova A, et al. Fullerenes as anti-aging antioxidants. Curr Aging Sci. 2017;10(1):56-67.
Gerber PA, Rutter GA. The Role of oxidative stress and hypoxia in pancreatic beta-cell dysfunction in diabetes mellitus. Antiox Redox Signal. 2017;26(10):501-518.
Gholizadeh F, Mokarram P, Dastgheib S, Rahpeima Z. The effect of the aquatic extract of stevia on the MDA level and catalase activity in the testicular tissue of streptozotocin-nicotinamide-induced diabetic rats. Shiraz E-Med J. 2018;19(9):61044.
Ghorbani Z, Farahani RM, Aliaghaei A, Khodagholi F, Houssein Meftahi G, Danyali S, et al. Resveratrol protects purkinje neurons and restores muscle activity in rat model of cerebellar Ataxia. J Mol Neurosci. 2018;65(1):35-42.
Hadipour M, Kaka G, Bahrami F, Meftahi GH, Pirzad Jahromi G, Mohammadi A, et al. Crocin improved amyloid beta induced long-term potentiation and memory deficits in the hippocampal CA1 neurons in freely moving rats. Synapse. 2018;72(5):22026.
Horal M, Zhang Z, Stanton R, Virkamäki A, Loeken MR. Activation of the hexosamine pathway causes oxidative stress and abnormal embryo gene expression: Involvement in diabetic teratogenesis. Clin Mol Teratol. 2004;70(8):519-527.
Jiang YL, Ning Y, Ma XL, Liu YY, Wang Y, Zhang Z, et al. Alteration of the proteome profile of the pancreas in diabetic rats induced by streptozotocin. Int J Mol Med. 2011;28(2):153-160.
Kolb H. Mouse models of insulin dependent diabetes: low-dose streptozocin-induced diabetes and nonobese diabetic (NOD) mice. Diabetes Metab Rev. 1987;3(3):751-778.
Lin AM, Fang SF, Lin SZ, Chou CK, Luh TY, Ho LT. Local carboxyfullerene protects cortical infarction in rat brain. Neurosci Res. 2002;43(4):317-321.
Montano ME, Molpeceres V, Mauriz JL, Garzo E, Cruz IB, González P, et al. Effect of melatonin supplementation on food and water intake in streptozotocin-diabetic and non-diabetic male Wistar rats. Nutr Hosp. 2010;25(6):931-938.
Mori T, Takada H, Ito S, Matsubayashi K, Miwa N, Sawaguchi T. Preclinical studies on safety of fullerene upon acute oral administration and evaluation for no mutagenesis. Toxicology . 2006;225(1):48-54.
Mousavi SZ, Nafisi S, Maibach HI. Fullerene nanoparticle in dermatological and cosmetic applications. Nanomedicine. 2017;13(3):1071-1087.
Mutavdzin S, Gopcevic K, Stankovic S, Jakovljevic Uzelac J, Labudovic Borovic M, Djuric D. The effects of folic acid administration on cardiac oxidative stress and cardiovascular biomarkers in diabetic rats. Oxid Med Cell Longev. 2019;2019:1342549.
Namdar F, Bahrami F, Bahari Z, Ghanbari B, Elahi SA, Mohammadi MT. Evaluation of the effects of fullerene C60 nanoparticles on oxidative stress parameters in normal rats liver and brain. J Adv Med Biomed Res. 2019;27(124):8-15.
Namdar F, Shahyad S, Bahrami F, Bahari Z, Mohammadi MT. Application of fullerene nanoparticles to improve brain health and prevent neuronal damages in diabetes mellitus; a review study. Healt Res J. 2020;5(2):110-117.
Oeckinghaus A, Hayden MS, Ghosh S. Crosstalk in NF-κB signaling pathways. Nat Immunol. 2011;12(8):695-708.
Partha R, Conyers JL. Biomedical applications of functionalized fullerenebased nanomaterials. Int J Nanomed. 2009;4:261-275.
Rasouli Vani J, Mohammadi MT, Sarami Foroshani M, Rezazade E. Evaluation of the neuroprotective and antioxidant effects of Dorema aucheri extract on cerebral ischaemia-reperfusion injury in rats. Pharm Biol. 2019;57(1):255-262.
Sandireddy R, Yerra VG, Areti A, Komirishetty P, Kumar A. Neuroinflammation and oxidative stress in diabetic neuropathy: futuristic strategies based on these targets. Int J Endocrin. 2014;2014:674987.
Sheweita SA, Newairy AA, Mansour HA, Yousef MI. Effect of some hypoglycemic herbs on the activity of phase I and II drug-metabolizing enzymes in alloxan-induced diabetic rats. Toxicology . 2002;174(2):131-139.
Sheweita SA, Mashaly S, Newairy AA, Abdou HM, Eweda SM. Changes in oxidative stress and antioxidant enzyme activities in streptozotocin-induced diabetes mellitus in rats: role of alhagi maurorum extracts. Oxid Med Cell Longev. 2016;2016:5264064.
Sugeçti S. Role of protein oxidation, lipid peroxidation and antioxidant defense systems on diabetes mellitus. A J Health Sci. 2018;1(1):47-54.
Talebanzadeh S, Ashrafi M, Kazemipour N, Erjaee H, Nazifi S. Evaluation of the effects of saffron aqueous extract on oxidative stress in the lens of streptozotocin-induced diabetic rats. Biomed Res Ther. 2018;5(4):2133-2141.
Tangvarasittichai S. Oxidative stress, insulin resistance, dyslipidemia and type 2 diabetes mellitus. World J Diabetes. 2015;6(3):456.
Tietz F. Enzymic method for quantitatve determination of nanogram amount of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem . 1969;27(3):502-522.
Tsachouridis S, Papaioannidou P. Fullerenes: Chemical structure and properties. Front Pharmacol 2010, Conference Abstract: 8th Southeast European Congress on Xenobiotic Metabolism and Toxicity.
Wautier MP, Guillausseau PJ, Wautier JL. Activation of the receptor for advanced glycation end products and consequences on health. Diabetes Metab Syndr. 2017;11(4):305-309.
Ye S, Chen M, Jiang Y, Chen M, Zhou T, Wang Y, et al. Polyhydroxylated fullerene attenuates oxidative stress-induced apoptosis via a fortifying Nrf2-regulated cellular antioxidant defence system. Int J Nanomed . 2014;9:2073-2087.
Yi JK, Ryoo ZY, Ha JJ, Oh DY, Kim MO, Kim SH. Beneficial effects of 6-shogaol on hyperglycemia, islet morphology and apoptosis in some tissues of streptozotocin-induced diabetic mice. Diabetol Metab Syndr. 2019;11(1):1-3.
Zha YY, Yang B, Tang ML, Guo QC, Chen JT, Wen LP, et al. Concentration-dependent effects of fullerenol on cultured hippocampal neuron viability. Int J Nanomedicine . 2012;7:3099-3109.
Zlatkis A, Zak B, Boyle AJ. A new method for the direct determination of serum cholesterol. J Lab Clin Med. 1953;41(3):486-492.
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Baqiyatallah University of Medical Sciences
Grant numbers IR.BMSU.REC.1399.332
