Development and validation of a simple and noninvasive method for salivary uric acid: potential applications for monitoring the salivary uric acid level in healthy volunteers and gout patients

Li-Li  Zhao; Qingkun  Ma; Chun-Yan  Xu; Guanghao  Zheng; Yu Han; Qing  Qian; Wenlong  Jin; Jun Zhe Min

doi:10.1590/s2175-97902022e201026

Authors

Li-Li Zhao Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, Yanji, People’s Republic of China
Qingkun Ma Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, Yanji, People’s Republic of China
Chun-Yan Xu Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, Yanji, People’s Republic of China
Guanghao Zheng Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, Yanji, People’s Republic of China
Yu Han Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, Yanji, People’s Republic of China
Qing Qian Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, Yanji, People’s Republic of China
Wenlong Jin Department of Endocrinology, Yanbian University Hospital, Yanji, People’s Republic of China
Jun Zhe Min Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Pharmaceutical Analysis, College of Pharmacy Yanbian University, Yanji, People’s Republic of China https://orcid.org/0000-0002-9581-2022

DOI:

https://doi.org/10.1590/s2175-97902022e201026

Keywords:

Noninvasive, Gout patient, Hyperuricemia, Human saliva, Uric acid

Abstract

Serum uric acid (UA) is a traditional biomarker in the clinical diagnosis of gout and hyperuricemia. However, serum treatment and storage are cumbersome, and wounds are susceptible to infection. Therefore, in this study, a simple and noninvasive method was developed to detect the UA in human saliva to monitor the gout. An Inertsil ODS-3 column was used for the analysis under the condition of isocratic elution with the mixed solution phosphate buffer (74 mM, pH=2.2): Methanol=98:2 (v:v) and the UV detection at 284 nm. Using salivary UA data from healthy volunteers (HVs) (n=68) and gout patients (GPs) (n=14), we examined the salivary UA difference in their content. The intra-and inter-day accuracy and precision (RSD %) were less than 2.56%, the limit of detection (LOD) of UA was 5.0 ng/mL, the mean recoveries of the corresponding compounds were 102.48%. Saliva levels of UA in HVs and GPs were 35.26±14.06 µg/mL and 91.96±23.90 µg/mL, respectively. The concentrations of salivary UA in GPs were significantly higher than those in HVs ( p < 0.001). This method was also expected to monitor the hyperuricemia and other metabolic disorders in the future.

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Development and validation of a simple and noninvasive method for salivary uric acid

potential applications for monitoring the salivary uric acid level in healthy volunteers and gout patients

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