In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization
DOI:
https://doi.org/10.1590/1678-7757-2016-0513Keywords:
X-ray microtomography, Tooth remineralization, Calcium phosphates, Fluoride, Dental enamelAbstract
Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.Downloads
Download data is not yet available.
Downloads
Published
2017-12-01
Issue
Section
Original Articles
License
Todo o conteúdo do periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons do tipo atribuição CC-BY.
How to Cite
In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization. (2017). Journal of Applied Oral Science, 25(6), 596-603. https://doi.org/10.1590/1678-7757-2016-0513