Natural regeneration in Atlantic Forest Fragments: using ants (Hymenoptera: Formicidae) for monitoring a conservation unit


  • Fabrício Severo Magalhães Universidade de Mogi das Cruzes, Núcleo de Ciências Ambientais, Laboratório de Mirmecologia do Alto Tietê. Mogi das Cruzes, SP, Brasil.
  • Débora Rodrigues de Souza Campana Universidade de Mogi das Cruzes, Núcleo de Ciências Ambientais, Laboratório de Mirmecologia do Alto Tietê. Mogi das Cruzes, SP, Brasil.
  • Gisely da Silva Freitas Universidade de Mogi das Cruzes, Núcleo de Ciências Ambientais, Laboratório de Mirmecologia do Alto Tietê. Mogi das Cruzes, SP, Brasil.
  • Marlina Ribeiro Abonizio-Santos Universidade de Mogi das Cruzes, Núcleo de Ciências Ambientais, Laboratório de Mirmecologia do Alto Tietê. Mogi das Cruzes, SP, Brasil.
  • Lívia Pires do Prado Universidade de São Paulo, Museu de Zoologia. São Paulo, SP, Brasil.
  • Maria Santina de Castro Morini Universidade de Mogi das Cruzes, Núcleo de Ciências Ambientais, Laboratório de Mirmecologia do Alto Tietê. Mogi das Cruzes, SP, Brasil.



Species inventory, Biological Conservation, Richness, Environmental Reserve, Biodiversity


The Brazilian Atlantic Forest is considered one of the most threatened tropical forests in the world due to the extensive environmental impact it has endured throughout history. Only 12.4% of its original vegetation is estimated to remain. Even though reduced and highly fragmented, it houses enormous biodiversity, and its preservation is paramount to the maintenance of the country’s fauna, flora, funga and microbiota. One of the most efficient measures adopted by public agencies aimed at protecting biodiversity has been the creation of conservation units. To evaluate the preservation state of protected areas, several environmental studies have been performed; species inventories are one among them. Ants are excellent bioindicators, for they are not only sensitive to environmental changes, but they also have a history of being used in impact assessment (i.e., fragmentation). In this study we assessed the ant communities inhabiting the leaf litter in areas with different regeneration states at the RPPN Botujuru – Serra do Itapety (Mogi das Cruzes, São Paulo – Brazil). Mini-Winkler traps were used in the ant survey, and diversity analyses were performed. In total, we recorded 86 species of ants, with a highlight to Camponotus cillae Forel, 1912, a species that remained unrecorded for the state of São Paulo for over 100 years, and a possible new species of Octostruma Forel, 1912. Overall, the species found show that the areas are in the process of natural regeneration. Our data on RPPN Botujuru is unprecedented, and our species list has the potential of being used as an effective monitoring tool for this conservation unit.


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Agosti, D.; Majer, J.D.; Alonso, L.E. & Schultz, T.R. 2000. Ants: standard methods for measuring and monitoring biodiversity. Washington, Smithsonian Institution Press.

Aguiar, J.J.M.; Anjos, D.V.; Carvalho, R.L.; Rejane de Almeida, W.; Santos; A.C.C. & Santos, J.C. 2022. Plant richness drives ant diversity in Eucalyptus‐dominated landscape on Brazilian savanna. Austral Ecology, 47: 17-25.

Ayala, L. 2010. RPPN Mata Atlântica: Empresas aliadas da natureza RPPN Mata Atlântica as reservas particulares como estratégia ambiental corporativa. São Paulo, SOS Mata Atlântica,.

Baccaro, F.B.; Feitosa, R.M.; Fernández, F.; Fernandes, I.O.; Izzo, T.J.; Souza, J.L.P. & Solar, R. 2015. Guia para os gêneros de formigas do Brasil. Manaus, Editora INPA,.

Baklanov, A.; Grimmond, C.S.B.; Carlson, D.; Terblanche, D.; Tang, X.; Bouchet, V.; Lee, B.; Langendijk, G.; Kolli, R.K. & Hovsepyan, A. 2018. From urban meteorology, climate and environment research to integrated city services. Urban Climate, 23: 330-341.

Barbosa, L.P. & Fernandes, W.D. 2003. Bait removal by ants (Hymenoptera: Formicidae) in managed and unmanaged Eucalyptus urophylla ST Blake Fields. Brazilian Journal of Ecology, 8: 61-63.

Benayas, J.M.R.; Bullock, J.M. & Newton, A.C. 2008. Creating woodland islets to reconcile ecological restoration, conservation, and agricultural land use. Frontiers in Ecology and the Environment, 6(6), 329-336.

Bensusan, N. 2006. Conservação da biodiversidade em áreas protegidas. Rio de Janeiro, FGV Editora.

Bolton, B. 2022. An online catalog of the ants of the world. Available: Access: 27/09/2022.

Borowiec, M.L. 2016. Generic revision of the ant subfamily Dorylinae (Hymenoptera, Formicidae). ZooKeys, 608: 1-280.

Brandão, C.R.F. 1999. Reino Animalia: Formicidae. In: Joly, C.A. & Cancello, E.M. Biodiversidade do Estado de São Paulo: síntese do conhecimento ao final do século XX: Invertebrados. São Paulo, FAPESP. p. 1-300.

Brandão, C.R.F.; Prado, L.P.; Ulysséa, M.A.; Probst, R.S. & Alarcon, V. 2015. Dieta das Poneromorfas Neotropicais. In: Delabie, J.H.C; Feitosa, R.M.; Serrão, J.E.; Mariano, C.S.F.; Majer, J.D. As formigas Poneromorfas do Brasil. Ilhéus, Editora da UESC. p. 145-161.

Brandão, C.R.F.; Silva, R.R. & Delabie, J.H.C. 2009. Formigas (Hymenoptera). In: Panizzi, A.R. & Parra, J.R.P. Bioecologia e nutrição de insetos: base para o manejo integrado de pragas. Brasília, Embrapa Tecnológica. 1163p.

Brasil. 2000. Lei Federal № 9.985, de 18 de julho de 2000. Institui o Sistema Nacional de Unidades de Conservação e dá outras providências. Available: Access: 21/08/2020.

Instituto Terra. 2022. Dia Nacional das RPPNs: o que é isso? Available: Access: 25/10/2022.

Brito, F. 2012. Corredores ecológicos: uma estratégia integradora na gestão de ecossistemas. Florianópolis, Editora UFSC.

Brown, W.L. 1957. Predation of arthropod eggs by the ant genera Proceratium and Discothyrea. Psyche: A Journal of Entomology, 64(3): 115-115.

Camacho, G.P.; Franco, W. & Feitosa, R.M. 2020. Additions to the taxonomy of Gnamptogenys Roger (Hymenoptera: Formicidae: Ectatomminae) with an updated key to the New World species. Zootaxa, 4747(3): 450-476.

Camacho, G.P.; Franco, W.; Branstetter, M.G.; Pie, M.R.; Longino, J.T.; Schultz, T.R. & Feitosa, R.M. 2022. UCE phylogenomics resolves major relationships among ectaheteromorph ants (Hymenoptera: Formicidae: Ectatomminae, Heteroponerinae): a new classification for the subfamilies and the description of a new genus. Insect Systematics and Diversity, 6(1): 1-20.

Campos, R.B.; Schoereder, J.H. & Sperber, C.F. 2003. Local determinants of species richness in litter ant communities (Hymenoptera: Formicidae). Sociobiology, 41(2): 357-368.

Carlucci, M.B.; Marcilio-Silva, V. & Torezan, J.M. 2021. The southern Atlantic Forest: use, degradation, and perspectives for conservation. In: Marcia, C.M.; Carlos, E. & Grelle, V. The Atlantic Forest. Cham, Springer. p. 91-111.

Centro de Previsão de Tempo e Estudos Climáticos (CPTEC)/Instituto Nacional de Pesquisas Espaciais (INPE). 2022. Available: Access: 15/02/2022.

Cheung, K.C.; Liebsch, D. & Marques, M.C.M. 2010. Forest recovery in newly abandoned pastures in Southern Brazil: implications for the Atlantic Rain Forest resilience. Natureza & Conservação, 8(1): 66-70.

Chu, S.; Ouyang, J.; Liao, D.; Zhou, Y.; Liu, S.; Shen, D.; Wei, X. & Zeng, S. 2019. Effects of enriched planting of native tree species on surface water flow, sediment, and nutrient losses in a Eucalyptus plantation forest in southern China. Science of the Total Environment, 675: 224-234.

Colombo, A.F. & Joly C.A. 2010. Brazilian Atlantic Forest lato sensu: the most ancient Brazilian forest, and a biodiversity hotspot, is highly threatened by climate change. Brazilian Journal of Biology, 70(3): 697-708.

Couto, P.H.M.; Araújo, M.S.; Rodrigues, O.S.; Lucia, T.M.C.D.; Oliveira, M.A. & Bacci, L. 2010. Formigas como bioindicadores da qualidade ambiental em diferentes sistemas de cultivo da soja. Revista Agrotecnologia, 1(1): 11-20.

Cramer, M.J. & Willig, M.R. 2005. Habitat heterogeneity, species diversity and null models. Oikos, 108(2): 209-218.

Dejean, A. & Dejean, A. 1998. How a ponerine ant acquired the most evolved mode of colony foundation. Insectes Sociaux, 45(3): 343-346.

Delabie, J.H.; Agosti, D. & Nascimento, I.D. 2000. Litter ant communities of the Brazilian Atlantic rain forest region. In: Agosti, D.; Majer, J.; Alonso, L.; Schultz, T. Sampling Ground-dwelling Ants: case studies from the world’s rain forests. Perth, Curtin University of Technology School of Environmental Biology.

Ecofuturo. 2016. Instituto Ecofuturo. Reserva Botujuru Serra do Itapety: Um breve resumo do plano de manejo. São Paulo.

Fernandes, I.O.; Larabee, F.J.; Oliveira, M.L.; Delabie, J.H. & Schultz, T.R. 2021. A global phylogenetic analysis of trap‐jaw ants, Anochetus Mayr and Odontomachus Latreille (Hymenoptera: Formicidae: Ponerinae). Systematic Entomology, 46(3): 685-703.

Fernández, F. 2003. Subfamília Formicinae. In: Fernández, F. (Ed.). Introduccion a las hormigas de la región Neotropical. Bogotá, Instituto de Investigación de Recursos Biológicos Alexander Von Humbolt, p. 299-306.

Fonseca, R.C. & Diehl, E. 2004. Riqueza de formigas (Hymenoptera, Formicidae) epigéicas em povoamentos de Eucalyptus spp. (Myrtaceae) de diferentes idades no Rio Grande do Sul, Brasil. Revista Brasileira de Entomologia, 48(1): 95-100.

Forel, A. 1912. Formicides néotropiques. Part VI. 5me sous-famille Camponotinae Forel. Mémoires de la Société Entomologique de Belgique, 20: 59-92.

Forister, M.L.; Pelton, E.M. & Black, S.H. 2019. Declines in insect abundance and diversity: We know enough to act now. Conservation Science and Practice, 1-8.

Franco, W.; Ladino, N.; Delabie, J. H.; Dejean, A.; Orivel, J.; Fichaux, M.; Groc, S.; Leponce, M. & Feitosa, R.M. 2019. First checklist of the ants (Hymenoptera: Formicidae) of French Guiana. Zootaxa, 4674(5): 509-543.

Fittkau, E.J. & Klinge, H. 1973. On biomass and trophic structure of the central Amazonian rain forest ecosystem. Biotropica, 5(1): 2-14.

Gardner, T.A.; Barlow, J.; Chazdon, R.; Ewers, R.M.; Harvey, C.A.; Peres, C.A. & Sodhi, N.S. 2009. Prospects for tropical forest biodiversity in a human‐modified world. Ecology Letters, 12(6): 561-582.

Gerhardt, A. 2002. Bioindicator species and their use in biomonitoring. Environmental Monitoring, 1: 77-123.

Gotelli, N. & Colwell, R.K. 2001. Quantifying biodiversity: procedures and pitfalls in the measurement and comparison of species richness. Ecology Letters, 4(4): 379-391.

Grimbacher, P.S. & Hughes, L. 2002. Response of ant communities and ant-seed interactions to bush regeneration. Ecological Management & Restoration, 3(3): 188-199.

Hammer, O.; Harper, D.A.T. & Ryan P.D. 2001. PAST: Paleontological statistics software package for education and data analysis. Paletontologia Eletronica, 4(1): 1-9.

Hölldobler, B. & Wilson, E.O. 1990. The ants. Cambridge, Belknap Press.

Hu, X.; Huang, B.; Verones, F.; Cavalett, O. & Cherubinil, F. 2021. Overview of recent land‐cover changes in biodiversity hotspots. Frontiers in Ecology and the Environment, 19(2): 91-97.

Janicki, J.; Narula, N.; Ziegler, M.; Guénard, B. & Economo, E.P. 2016. Visualizing and interacting with large-volume biodiversity data using client-server web-mapping applications: The design and implementation of Ecological Informatics, 32: 185-193.

Kaspari, M. 1996. Testing resource-based models of patchiness in four Neotropical litter ant assemblages. Oikos, 443-454.

Katayama, M. 2013. Predatory behaviours of Discothyrea kamiteta (Proceratiinae) on spider eggs. Asian Myrmecol, (5): 121-124.

Laurance, W.F.; Nascimento, H.E.; Laurance, S.G.; Andrade, A.C.; Fearnside, P.M.; Ribeiro, J.E. & Capretz, R.L. 2006. Rain forest fragmentation and the proliferation of successional trees. Ecology, 87(2): 469-482.

Le Saout, S.; Hoffmann, M.; Shi, Y.; Hughes, A.; Bernard, C.; Brooks, T. M.; Bertzky, B.; Butchart, S.H.M.; Stuart, S.N.; Badman, T. & Rodrigues, A.S.L. 2013. Protected areas and effective biodiversity conservation. Science, 342(6160): 803-805.

Lewis, E.; MacSharry, B.; Juffe‐Bignoli, D.; Harris, N.; Burrows, G.; Kingston, N. & Burgess, N.D. 2019. Dynamics in the global protected‐area estate since 2004. Conservation Biology, 33(3): 570-579.

Lima, R.A.; Oliveira, A.A.; Pitta, G.R.; Gasper, A.L.; Vibrans, A.C.; Chave, J.; Steege, H.T. & Prado, P.I. 2020. The erosion of biodiversity and biomass in the Atlantic Forest biodiversity hotspot. Nature Communications, 11(1): 1-16.

Lima, P.C.A. & Franco, J.L.A. 2014. As RPPNs como estratégia para a conservação da biodiversidade: o caso da Chapada dos Veadeiros. Sociedade & Natureza, 26: 113-125.

Liu, J.; Slik, F.; Zheng, S. & Lindenmayer, D.B. 2022. Undescribed species have higher extinction risk than known species. Conservation Letters, e12876.

MacDicken, K.G. 2015. Global forest resources assessment 2015: what, why and how? Forest Ecology and Management, 352: 3-8.

Majer, J.D. & Delabie, J.H.C. 1999. Impact of tree isolation on arboreal and communities in cleared pasture in the Atlantic rain forest region of Bahia, Brazil. Insect Sociaux, 46: 281-290.

Marques, M.C.M. & Grelle, C.E.V. 2021. The Atlantic Forest: History, Biodiversity, Threats and Opportunities of the Mega-diverse Forest. Cham, Springer Nature.

Martello, F.B.; Morini, M.S.C.; Silva, R.R.; Souza-Campana, D.R.; Ribeiro, M.C. & Carmona, C.P. 2018. Homogenization and impoverishment of taxonomic and functional diversity of ants in Eucalyptus plantations. Scientific Reports, 8(1): 3266.

Martins, L.; Almeida, F.S.; Mayhé-Nunes, A.J. & Vargas A.B. 2011. Efeito da complexidade estrutural do ambiente sobre as comunidades de formigas (Hymenoptera: Formicidae) no município de Resende, RJ, Brasil. Revista Brasileira de Biociências, 9(2): 174-179.

Medeiros, R.; Young, C.E.F.; Pavese, H.B. & Araújo, F.F.S. 2011. Contribuição das unidades de Conservação Brasileiras para a economia Nacional. Brasília, UNEP-WCMC.

Melo, F.P.; Arroyo-Rodríguez, V.; Fahrig, L.; Martínez-Ramos, M. & Tabarelli, M. 2013. On the hope for biodiversity-friendly tropical landscapes. Trends in Ecology & Evolution, 28(8): 462-468.

Mentone, T.D.O.; Diniz, E.A.; Munhae, C.D.B.; Bueno, O.C. & Morini, M.S.C. 2011. Composição da fauna de formigas (Hymenoptera: Formicidae) de serapilheira em florestas semidecídua e de Eucalyptus spp., na região sudeste do Brasil. Biota Neotropica, 11: 237-246.

Ministério do Meio Ambiente (MMA). 2022. Mata Atlântica. Available: Access: 13/03/2022.

Mogi das Cruzes. 2020. Plano municipal de conservação e recuperação da Mata Atlântica. Prefeitura de Mogi das Cruzes. Available: Access: 26/09/2022.

Morini, M.S.C. & Miranda, V.F.O. 2012. Serra do Itapety: Aspectos históricos, sociais e naturalísticos. Bauru, Canal 6.

Myers, N.; Mittermeier, R.A.; Mittermeier, C.G.; Fonseca, G.A.B. & Kent, J. 2000. Biodiversity hotspots for conservation priorities. Nature, 403(6772): 853-858.

Ortiz-Sepulveda, C.M.; Bocxlaer, B.V; Meneses, A.D. & Fernández, F. 2019. Molecular and morphological recognition of species boundaries in the neglected ant genus Brachymyrmex (Hymenoptera: Formicidae): toward a taxonomic revision. Organisms Diversity & Evolution, 19(3): 447-542.

Pacheco, R.; Silva, R.R.; Morini, M.S.D.C. & Brandão, C.R. 2009. A comparison of the leaf-litter ant fauna in a secondary atlantic forest with an adjacent pine plantation in southeastern Brazil. Neotropical Entomology, 38(1): 55-65.

Palacio, E.E. 2019. Subfamilia Dorylinae. In: Fernández, F. & Guerrero, R.J.; Delsinne, T. Hormigas de Colombia. Bogotá, Universidad Nacional de Colombia. p. 571-630.

Pardini, R.; Faria, D.; Accacio, G.M.; Laps, R.R.; Mariano-Neto, E.; Paciencia, M.L.B.; Dixo, M. & Baumgarten, J. 2009. The challenge of maintaining Atlantic Forest biodiversity: a multi-taxa conservation assessment of specialist and generalist species in an agro-forestry mosaic in southern Bahia. Biological Conservation, 142(6): 1178-1190.

Pereira, M.P.S.; Queiroz, J.M.; Souza, G.O. & MayhéNunes, A.J. 2007. Influência da heterogeneidade da serapilheira sobre as formigas que nidificam em galhos mortos em floresta nativa e plantio de eucalipto. Neotropical Biology and Conservation, 2(3): 161-164.

Pinto, L.P.; Hirota, M.; Guimarães, E.; Fonseca, M.; Martinez, D.I. & Takahashi, C.K. 2017. Unidades de Conservação Municipais da Mata Atlântica. São Paulo, SOSMA.

Rezende, C.L.; Scarano, F.R.; Assad, E.D.; Joly, C.A.; Metzfer, J.P.; Strassuburg, B.B.N.; Tabarelli, M.; Fonseca, G.A. & Mittermeier, R.A. 2018. From hotspot to hopespot: An opportunity for the Brazilian Atlantic Forest. Perspectives in Ecology and Conservation, 16(4): 208-2014.

Ribas, C.R.; Campos, R.B.; Schmidt, F.A. & Solar, R.R. 2012. Ants as indicators in Brazil: a review with suggestions to improve the use of ants in environmental monitoring programs. Psyche: A Journal of Entomology, 4(6): 1-23.

Rosumek, F.B. 2017. Natural history of ants: what we (do not) know about trophic and temporal niches of neotropical species. Sociobiology, 64(3): 244-255.

Secretaria de Infraestrutura e Meio Ambiente do Estado de São Paulo (SIMA/SP). 2019. Estado de São Paulo ganha quatro novas reservas particulares de patrimônio natural. Available: Access: 25/10/2022.

Silva, R.R. & Brandão, C.R.F. 2010. Morphological patterns and community organization in leaf-litter ant assemblages. Ecological Monographs, 80: 107-124.

Sobral-Souza, T.; Vancine, M.H.; Ribeiro, M.C. & Lima-Ribeiro, M.S. 2018. Efficiency of protected areas in Amazon and Atlantic Forest conservation: A spatio-temporal view. Acta Oecologica, 87: 1-7.

Souza-Campana, D.R.; Wazema, C.T.; Magalhães, F.S.; Silva, N.S.; Nagatani, V.H.; Suguituru, S.S.; Goto, M.A. & Morini, M.S.C. 2020. Coleção de referência do Laboratório de Mirmecologia do Alto Tietê, São Paulo, Brasil: status atual e perspectivas. Boletim do Museu Paraense Emílio Goeldi-Ciências Naturais, 15(1): 317-336.

Suguituru, S.S.; Morini, M.S.C.; Feitosa, R.M. & Silva, R.R. 2015. Formigas do Alto Tietê. Bauru, Canal 6.

Suguituru, S.S.; Silva, R.R.; Souza, D.R.D.; Munhae, C.D.B. & Morini, M.S.C. 2011. Ant community richness and composition across a gradient from Eucalyptus plantations to secondary Atlantic Forest. Biota Neotropica, 11(1): 369-376.

Suguituru, S.S.; Souza, D.R.D.; Munhae, C.D.B; Pacheco, R. & Morini, M.S.D.C. 2013. Diversidade e riqueza de formigas (Hymenoptera: Formicidae) em remanescentes de Mata Atlântica na Bacia Hidrográfica do Alto Tietê, SP. Biota Neotropica, 13: 141-152.

Underwood, E.C. & Fisher, B.L. 2006. The role of ants in conservation monitoring: if, when, and how. Biological conservation, 132(2): 166-182.

Vasconcelos, H.L.; Vilhena, J.M.S. & Caliri, G.J.A. 2000. Responses of ants to selective logging of a central Amazonian Forest. Journal of Applied Ecology, 37(3): 508-514.

Viles, H.A; Goudie, A.S.; & Goudie, A.M. 2021. Ants as geomorphological agents: A global assessment. Earth-Science Reviews, 213: 1-17, 103469.

Wazema, C.T.; Morini, M.S.C. & Souza-Campana, D.R. 2019. Diversidade de formigas (Hymenoptera: Formicidae) em um fragmento de Mata Atlântica no município de Mogi das Cruzes (SP). Revista Científica UMC, 4(2): 1-12.

Wazema, C.T.; Nagatani, V.H.; Souza-Campana, D.R.; Magalhães, F.S.; Sartorello, R. & Morini, M.S.C. 2020. What do different landscapes of the Atlantic Forest reveal about the occurrence of Discothyrea Roger, 1863 (Formicidae: Proceratiinae)? Biota Neotropica, 20(4).

Wiedmann, S.M. 2001. Reserva do patrimônio natural – RPPN – na Lei № 9.985/2000, que instituiu o sistema nacional de unidades de conservação – SNUC. Direito ambiental das áreas protegidas: o regime jurídico das unidades de conservação. Rio de Janeiro, Forense.

Wilson, E.O. 2003. Pheidole in the New World: a dominant, hyperdiverse ant genus. Cambridge, Harvard University Press. 794p.

Wilson, K.A.; Meijaard, E.R.I.K.; Drummond, S.; Grantham, H.S.; Boitani, L.; Catullo, G.; Christie, L.; Dennis, R.; Dutton, I.; Falcucci, A.; Maiorano, L.; Possingham, H.P.; Rondinni, C.; Turner, W.R.; Venter, O. & Watts, M. 2010. Conserving biodiversity in production landscapes. Ecological Applications, 20(6): 1721-1732.






Original Article

How to Cite

Magalhães, F. S., Campana, D. R. de S., Freitas, G. da S., Abonizio-Santos, M. R., Prado, L. P. do, & Morini, M. S. de C. (2022). Natural regeneration in Atlantic Forest Fragments: using ants (Hymenoptera: Formicidae) for monitoring a conservation unit. Papéis Avulsos De Zoologia, 62, e202262071.