Sporothrix schenckiiCOMPLEX:SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES

Authors

  • Daniele Carvalho OLIVEIRA Universidade Federal de Santa Maria; Departamento de Microbiologia e Parasitologia
  • Érico Silva de LORETO Universidade Federal de Santa Maria; Departamento de Microbiologia e Parasitologia
  • Débora Alves Nunes MARIO Universidade Federal de Santa Maria; Centro de Ciências da Saúde
  • Paulo G. Markus LOPES Universidade Federal de Santa Maria; Centro de Ciências da Saúde
  • Louise Vignolles NEVES Universidade Federal de Santa Maria; Departamento de Microbiologia e Parasitologia
  • Marta Pires da ROCHA Universidade Federal de Santa Maria; Departamento de Microbiologia e Parasitologia
  • Janio Morais SANTURIO Universidade Federal de Santa Maria; Departamento de Microbiologia e Parasitologia
  • Sydney Hartz ALVES Universidade Federal de Santa Maria; Centro de Ciências da Saúde

Abstract

Sporothrix schenckiiwas reclassified as a complex encompassing six cryptic species, which calls for the reassessment of clinical and epidemiological data of these new species. We evaluated the susceptibility of Sporothrix albicans(n = 1) , S. brasiliensis(n = 6) , S. globosa(n = 1), S. mexicana(n = 1) and S. schenckii(n = 36) to terbinafine (TRB) alone and in combination with itraconazole (ITZ), ketoconazole (KTZ), and voriconazole (VRZ) by a checkerboard microdilution method and determined the enzymatic profile of these species with the API-ZYM kit. Most interactions were additive (27.5%, 32.5% and 5%) or indifferent (70%, 50% and 52.5%) for TRB+KTZ, TRB+ITZ and TRB+VRZ, respectively. Antagonisms were observed in 42.5% of isolates for the TRB+VRZ combination. Based on enzymatic profiling, the Sporothrix schenckiistrains were categorized into 14 biotypes. Leucine arylamidase (LA) activity was observed only for S. albicansand S. mexicana. The species S. globosaand S. mexicanawere the only species without β-glucosidase (GS) activity. Our results may contribute to a better understanding of virulence and resistance among species of the genus Sporothrixin further studies.

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Published

2015-08-01

How to Cite

OLIVEIRA, D. C., LORETO, Érico S. de, MARIO, D. A. N., LOPES, P. G. M., NEVES, L. V., ROCHA, M. P. da, SANTURIO, J. M., & ALVES, S. H. (2015). Sporothrix schenckiiCOMPLEX:SUSCEPTIBILITIES TO COMBINED ANTIFUNGAL AGENTS AND CHARACTERIZATION OF ENZYMATIC PROFILES . Revista Do Instituto De Medicina Tropical De São Paulo, 57(4), 289-294. Retrieved from https://www.revistas.usp.br/rimtsp/article/view/105537

Issue

Section

Mycology