QUANTITATIVE VS. CONVENTIONAL PCR FOR DETECTION OF HUMAN ADENOVIRUSES IN WATER AND SEDIMENT SAMPLES

Authors

  • Rodrigo STAGGEMEIER Universidade Feevale
  • Marina BORTOLUZZI Universidade Feevale
  • Tatiana Moraes da Silva HECK Universidade Feevale
  • Fernando Rosado SPILKI Universidade Feevale
  • Sabrina Esteves de Matos ALMEIDA Universidade Feevale

Abstract

Human Adenoviruses (HAdV) are notably resistant in the environment. These agents may serve as effective indicators of fecal contamination, and may act as causative agents of a number of different diseases in human beings. Conventional polymerase chain reaction (PCR) and, more recently, quantitative PCR (qPCR) are widely used for detection of viral agents in environmental matrices. In the present study PCR and SYBR(r)Green qPCR assays were compared for detection of HAdV in water (55) and sediments (20) samples of spring and artesian wells, ponds and streams, collected from dairy farms. By the quantitative methodology HAdV were detected in 87.3% of the water samples and 80% of the sediments, while by the conventional PCR 47.3% and 35% were detected in water samples and sediments, respectively.

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Published

2015-08-01

How to Cite

STAGGEMEIER, R., BORTOLUZZI, M., HECK, T. M. da S., SPILKI, F. R., & ALMEIDA, S. E. de M. (2015). QUANTITATIVE VS. CONVENTIONAL PCR FOR DETECTION OF HUMAN ADENOVIRUSES IN WATER AND SEDIMENT SAMPLES . Revista Do Instituto De Medicina Tropical De São Paulo, 57(4), 299-303. Retrieved from https://www.revistas.usp.br/rimtsp/article/view/105540

Issue

Section

Virology