MOLECULAR INVESTIGATION OF HEMOTROPIC MYCOPLASMAS IN HUMAN BEINGS, DOGS AND HORSES IN A RURAL SETTLEMENT IN SOUTHERN BRAZIL

Authors

  • Rafael Felipe da Costa VIEIRA Universidade Federal do Paraná; Department of Veterinary Medicine
  • Odilon VIDOTTO Universidade Estadual de Londrina; Department of Preventive Veterinary Medicine
  • Thállitha Samih Wischral Jayme VIEIRA Universidade Estadual de Londrina; Department of Preventive Veterinary Medicine
  • Ana Márcia Sá GUIMARAES Purdue University; College of Veterinary Medicine; Department of Comparative Pathobiology
  • Andrea Pires dos SANTOS Purdue University; College of Veterinary Medicine; Department of Comparative Pathobiology
  • Naíla Cannes NASCIMENTO Purdue University; College of Veterinary Medicine; Department of Comparative Pathobiology
  • Nelson Jesse Rodrigues dos SANTOS Universidade Estadual de Londrina; Department of Preventive Veterinary Medicine
  • Thiago Fernandes MARTINS Universidade de São Paulo; Faculty of Veterinary Medicine; Department of Preventive Veterinary Medicine and Animal Health
  • Marcelo Bahia LABRUNA Universidade de São Paulo; Faculty of Veterinary Medicine; Department of Preventive Veterinary Medicine and Animal Health
  • Mary MARCONDES Universidade Estadual Paulista; Department of Veterinary Clinics, Surgery and Reproduction
  • Alexander Welker BIONDO University of Illinois; Department of Veterinary Pathobiology
  • Joanne Belle MESSICK Purdue University; College of Veterinary Medicine; Department of Comparative Pathobiology

Abstract

The aims of this study were to determine the prevalence of hemoplasmas in a rural Brazilian settlement's population of human beings, their dogs and horses, highly exposed to tick bites; to identify the tick species parasitizing dogs and horses, and analyze factors associated with their infection. Blood samples from 132 dogs, 16 horses and 100 humans were screened using a pan-hemoplasma SYBR green real-time PCR assay followed by a species-specific TaqMan real-time PCR. A total of 59/132 (44.7%) dog samples were positive for hemoplasmas (21 Mycoplasma haemocanisalone, 12 ' CandidatusMycoplasma haematoparvum' alone and 21 both). Only 1/100 (1.0%) human sample was positive by qPCR SYBR green, with no successful amplification of 16S rRNA or 23 rRNA genes despite multiple attempts. All horse samples were negative. Dogs >;1 year of age were more likely to be positive for hemoplasmas ( p= 0.0014). In conclusion, although canine hemoplasma infection was highly prevalent, cross-species hemoplasma transmission was not observed, and therefore may not frequently occur despite overexposure of agents and vectors.

Downloads

Download data is not yet available.

Downloads

Published

2015-08-01

How to Cite

VIEIRA, R. F. da C., VIDOTTO, O., VIEIRA, T. S. W. J., GUIMARAES, A. M. S., SANTOS, A. P. dos, NASCIMENTO, N. C., SANTOS, N. J. R. dos, MARTINS, T. F., LABRUNA, M. B., MARCONDES, M., BIONDO, A. W., & MESSICK, J. B. (2015). MOLECULAR INVESTIGATION OF HEMOTROPIC MYCOPLASMAS IN HUMAN BEINGS, DOGS AND HORSES IN A RURAL SETTLEMENT IN SOUTHERN BRAZIL. Revista Do Instituto De Medicina Tropical De São Paulo, 57(4), 353-357. Retrieved from https://www.revistas.usp.br/rimtsp/article/view/105550

Issue

Section

Bacteriology