Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods

Authors

  • José Figuerêdo-Silva Universidade Federal de Pernambuco; Laboratório de Imunopatologia Keizo Asami
  • Yoshimasa Kaneda Tokai University; School of Medicine; Department of Parasitology
  • Hiroshi Tachibana Tokai University; School of Medicine; Department of Parasitology
  • Rieko Furushima Juntendo University; School of Medicine; Department of Parasitology
  • Seiki Tateno Universidade Federal de Pernambuco; Laboratório de Imunopatologia Keizo Asami
  • Fernando Gomes Correia-Lima Universidade Federal do Piauí; Centro de Ciências da Saúde
  • Dalva Neves da Costa Bento Universidade Federal do Piauí; Centro de Ciências da Saúde

Keywords:

Trypanosoma cruzi, Epidemiological survey, Serodiagnosis, Indirect immunofluorescence, Enzyme-linked immunosorbent assay, Competitive enzyme-linked immunosorbent assay

Abstract

A survey of the prevalence of Trypanosoma cruzi infection was carried out in Oitis, a small community in the State of Piaui, Brazil. Two hundred and sixty five individuals were screened by microscopic examination, hémoculture, indirect immunofluorescence (IFA), enzyme-linked immunosorbent assay (ELISA), and competitive enzyme-linked immunosorbent assay (C-ELISA) using the monoclonal antibody TCF87 against to a 25kd T. cruzi antigen. Seropositivity was 14.3% by the IFA test, 14.7% by ELISA, and 13.2% by C-ELISA. The C-ELISA using the TCF87 monoclonal antibody seems to be applicable in serodiagnosis of Chagas' disease.

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Published

1991-06-01

Issue

Vol. 33 No. 3 (1991)

Section

Epidemiology

How to Cite

Figuerêdo-Silva, J., Kaneda, Y., Tachibana, H., Furushima, R., Tateno, S., Correia-Lima, F. G., & Bento, D. N. da C. (1991). Epidemiological survey of Trypanosoma cruzi infection in North-Eastern Brazil using different diagnostic methods . Revista Do Instituto De Medicina Tropical De São Paulo, 33(3), 193-198. https://www.revistas.usp.br/rimtsp/article/view/28818