Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA

Authors

  • Ana Maria Lissaldo USP; Instituto de Ciências Biomédicas
  • Sumie Hoshino-Shimizu Instituto Adolfo Lutz
  • Eufrosina Setsu Umezawa Instituto de Medicina Tropical de São Paulo
  • Anna Maria Simonsen Stolf USP; Instituto de Ciências Biomédicas

Keywords:

Chagas' disease, Epimastigote, Dot-ELISA, Serodiagnosis

Abstract

The alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) was assessed in dot-ELISA for the diagnosis of Chagas' disease. Serum samples (355) from chagasic and non-chagasic patients were studied, and IgG antibodies to ASEA were found in all patients with chronic Chagas' disease. In non-chagasic patients 95.6% were negative, except for those with leishmaniasis (visceral and mucocutaneous), and some patients from control group reacted in low titers. The data indicate that dot-ELISA using ASEA is suitable for seroepidemiologic surveys to be employed in endemic areas for Chagas' disease.

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Published

1994-04-01

Issue

Vol. 36 No. 2 (1994)

Section

Brief Communication

How to Cite

Lissaldo, A. M., Hoshino-Shimizu, S., Umezawa, E. S., & Stolf, A. M. S. (1994). Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to dot-ELISA . Revista Do Instituto De Medicina Tropical De São Paulo, 36(2), 163-166. https://www.revistas.usp.br/rimtsp/article/view/29141