Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report

Authors

  • Vanda Akico Ueda Fick de Souza Universidade de São Paulo; Faculdade de Medicina; Instituto de Medicina Tropical de São Paulo; Departamento de Doenças Infecciosas e Parasitárias
  • Laura Masami Sumita Universidade de São Paulo; Faculdade de Medicina; Instituto de Medicina Tropical de São Paulo; Departamento de Doenças Infecciosas e Parasitárias
  • Mary Eiko S. Otsubo Instituto Adolfo Lutz
  • Kioko Takei Universidade de São Paulo; Faculdade de Ciências Farmacêuticas
  • Cláudio Sérgio Pannuti Universidade de São Paulo; Faculdade de Medicina; Instituto de Medicina Tropical de São Paulo; Departamento de Doenças Infecciosas e Parasitárias

Keywords:

Rubella, ELISA, Antigen

Abstract

A simple method of rubella antigen production by treatment with sodium desoxycholate for use in enzyme immunoassay (IMT-ELISA) is presented. When this assay was compared with a commercial test (Enzygnost-Rubella, Behring), in the study of 108 sera and 118 filter paper blood samples, 96.9% (219/226) overall agreement and correlation coefficient of 0.90 between absorbances were observed. Seven samples showed discordant results, negative by the commercial kit and positive by our test. Four of those 7 samples were available, being 3 positive by HI.

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Published

1995-08-01

Issue

Vol. 37 No. 4 (1995)

Section

Preliminary Report

How to Cite

Souza, V. A. U. F. de, Sumita, L. M., Otsubo, M. E. S., Takei, K., & Pannuti, C. S. (1995). Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report . Revista Do Instituto De Medicina Tropical De São Paulo, 37(4), 357-359. https://www.revistas.usp.br/rimtsp/article/view/29290