Purification and parcial characterization of a hemagglutinating factor (HAF): a possible adhesive factor of the diffuse adherent of Escherichia coli (DAEC)

Authors

  • Yano Tomomasa University of Campinas; Institute of Biology; Department of Microbiology and Immunology
  • Cleide Ferreira Catani University of Campinas; Institute of Biology; Department of Microbiology and Immunology
  • Michiko Arita Osaka University; Research Institute for Microbiol Diseases; Department of Bacterial Infections
  • Takeshi Honda Osaka University; Research Institute for Microbiol Diseases; Department of Bacterial Infections
  • Toshio Miwatani Osaka University; Research Institute for Microbiol Diseases; Department of Bacterial Infections

Keywords:

Escherichia coli, Diffuse adherence, Hemagglutinating factor (HAF), adhesive factor

Abstract

The mannose-resistant hemagglutinating factor (HAF) was extracted and purified from a diffuse adherent Escherichia coli (DAEC) strain belonging to the classic enteropathogenic E. coli (EPEC) serotype (0128). The molecular weight of HAF was estimated to be 18 KDa by SDS-PAGE and 66 KDa by Sephadex G100, suggesting that the native form of HAF consists of 3-4 monomeric HAF. Gold immunolabeling with specific HAF antiserum revealed that the HAF is not a rigid structure like fimbriae on the bacterial surface. The immunofluorescence test using purified HAF on HeLa cells, in addition to the fact that the HAF is distributed among serotypes of EPEC, suggests that HAF is a possible adhesive factor of DAEC strains

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Published

1996-12-01

Issue

Vol. 38 No. 6 (1996)

Section

Microbiology

How to Cite

Tomomasa, Y., Catani, C. F., Arita, M., Honda, T., & Miwatani, T. (1996). Purification and parcial characterization of a hemagglutinating factor (HAF): a possible adhesive factor of the diffuse adherent of Escherichia coli (DAEC) . Revista Do Instituto De Medicina Tropical De São Paulo, 38(6), 401-406. https://www.revistas.usp.br/rimtsp/article/view/29410