Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat

Authors

  • Luciana Ruschel dos SANTOS Federal University of Rio Grande do Sul; Centre for Diagnostics and Research in Avian Pathology
  • Vladimir Pinheiro do NASCIMENTO Federal University of Rio Grande do Sul; Centre for Diagnostics and Research in Avian Pathology
  • Sílvia Dias de OLIVEIRA Federal University of Rio Grande do Sul; Centre for Diagnostics and Research in Avian Pathology
  • Maristela Lovato FLORES Federal University of Santa Maria
  • Alexandre Pontes PONTES Federal University of Rio Grande do Sul; Centre for Diagnostics and Research in Avian Pathology
  • Aldemir Reginato RIBEIRO Federal University of Rio Grande do Sul; Centre for Diagnostics and Research in Avian Pathology
  • Carlos Tadeu Pippi SALLE Federal University of Rio Grande do Sul; Centre for Diagnostics and Research in Avian Pathology
  • Rui Fernando Félix LOPES Federal University of Rio Grande do Sul; Centre of Biotechnology; Laboratory of Development and Production

Keywords:

Salmonella, PCR, Chicken meat

Abstract

The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10-7, 10-8 or 10-9 CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1%) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10-9 CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.

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Published

2001-10-01

Issue

Vol. 43 No. 5 (2001)

Section

Microbiology

How to Cite

SANTOS, L. R. dos, NASCIMENTO, V. P. do, OLIVEIRA, S. D. de, FLORES, M. L., PONTES, A. P., RIBEIRO, A. R., SALLE, C. T. P., & LOPES, R. F. F. (2001). Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat . Revista Do Instituto De Medicina Tropical De São Paulo, 43(5), 247-250. https://www.revistas.usp.br/rimtsp/article/view/30540