Molecular identification of Aspergillus spp. isolated from coffee beans
DOI:
https://doi.org/10.1590/S0103-90162005000100009Keywords:
ITS, ribosomal DNA, ochratoxin, mycotoxinAbstract
Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was the species most frequently detected, followed by A. ochraceus and A. carbonarius. The presence of A. carbonarius in immature coffee fruits harvested from trees is reported for the first time.Downloads
Download data is not yet available.
Downloads
Published
2005-01-01
Issue
Section
Genetics and Plant Breeding
License
All content of the journal, except where identified, is licensed under a Creative Common attribution-type BY-NC.How to Cite
Molecular identification of Aspergillus spp. isolated from coffee beans . (2005). Scientia Agricola, 62(1), 45-49. https://doi.org/10.1590/S0103-90162005000100009