Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids

Autores/as

  • Carlos Alexandre Gomes Ribeiro Federal University of Viçosa; Dept. of Genetics and Breeding
  • Marcos de Oliveira Pinto Embrapa Maize and Sorghum
  • Talles Eduardo Ferreira Maciel Federal University of Viçosa; Institute of Biotechnology Applied to Agriculture
  • Maria Marta Pastina Embrapa Maize and Sorghum
  • Everaldo Gonçalves de Barros Catholic University of Brasília; Dept. of Genomic Sciences and Biotecnhology
  • Claudia Teixeira Guimarães Embrapa Maize and Sorghum

DOI:

https://doi.org/10.1590/1678-992x-2016-0079

Palabras clave:

Zea mays, fingerprinting, genotyping, cultivar protection

Resumen

The development of efficient and low-cost genotyping methods is essential to precise genetic characterization of cultivars. Here, we present a system based on fluorescently labeled universal tail sequence primers (UTSP) to resolve microsatellite (SSR) markers as an alternative for molecular fingerprinting of maize. A set of 20 SSRs using the UTSP presented an average polymorphic information content of 0.84, which provided a probability of random identity ranging from 10−7 to 10−14, and a minimum exclusion power of 99.99998 % in a group of 48 tropical maize single-cross hybrids traded in Brazil. The genetic diversity analysis based on multidimensional scaling explained approximately 28 % of the total variance for the first two coordinates, tending to group the hybrids according to their respective origin. Additionally, this genotyping system presented a high distinctiveness capacity, which is widely recommended for genetic purity and fingerprinting analyses. Thus, this marker system has a strong potential as a tool for complementary analysis of distinguishability, uniformity and stability required for cultivar registration.

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Publicado

2017-04-01

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Cómo citar

Universal tail sequence-SSR applied to molecular characterization of tropical maize hybrids. (2017). Scientia Agricola, 74(2), 163-168. https://doi.org/10.1590/1678-992x-2016-0079