Milk macrophages from healthy female buffaloes: evaluation of phagocytosis test, spreading and hydrogen peroxide release assays

Authors

  • Alice Maria Melville Paiva Della Libera Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Eduardo Harry Birgel Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Sandra Satiko Kitamura Universidade Paulista, Faculdade de Medicina Veterinária, São Paulo, SP
  • Andrea Mello Franco Rosenfeld Laticínio Morro da Pedra
  • Ênio Mori Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Cristina de Oliveira Massoco-Salles Gomes Universidade Anhembi Morumbi, Faculdade de Medicina Veterinária, São Paulo, SP
  • Wanderley Pereira de Araújo Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26491

Keywords:

Buffalo, Milk, Leukocytes, Phagocytosis, Immunology

Abstract

The objective of the present study was to evaluate the functioning of nonelicited dairy macrophages present in phagocytosis, spreading and hydrogen peroxide release tests. Fifty-six samples of milk were collected from 15 healthy buffaloes. Total and differential somatic cell counts, cell viability, and indexes of phagocytosis, spreading and hydrogen peroxide release were assessed. The following values were obtained: mean of 14,500 cells/mL of milk, with median equal to 4.33% of lymphocytes; mean and standard deviation equal to 50.77% + 18.28 of monocytes / macrophages and 32.13% + 19.27 polymorphouclear cells. Mean viability of cells in suspension was 66.8% +15.8; phagocytosis and spreading indexes were equal to 30.1% + 16.9 and 58.5% +13.3, respectively. There was no difference between the spontaneous release of H2O2 and the one induced by PMA. It was concluded that nonelicited macrophages present in the milk of healthy buffaloes were significantly capable to spread and phagocyte. Phagocytes presented the ability to release hydrogen peroxide either spontaneously or not, in a maximum level and with a significant variation between samples.

Downloads

Download data is not yet available.

Published

2006-06-01

How to Cite

Della Libera, A. M. M. P., Birgel, E. H., Kitamura, S. S., Rosenfeld, A. M. F., Mori, Ênio, Gomes, C. de O. M.-S., & Araújo, W. P. de. (2006). Milk macrophages from healthy female buffaloes: evaluation of phagocytosis test, spreading and hydrogen peroxide release assays. Brazilian Journal of Veterinary Research and Animal Science, 43(3), 412-419. https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26491

Issue

Section

UNDEFINIED

Most read articles by the same author(s)

<< < 1 2 3 > >>