In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types

Authors

  • Lilian Mara Trevisan Tavares Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Weber Beringui Feitosa Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Viviani Purri de Oliveira Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Marcilio Nichi Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Mayra Elena Ortiz D'Ávila Assumpção Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • José Antonio Visintin Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP

DOI:

https://doi.org/10.11606/S1413-95962011000100005

Keywords:

Co-culture, Blastocyst, Granulosa, Oviduct, Vero, BRL

Abstract

The present work was carried out to evaluate the in vitro development of bovine embryo co-cultured in granulosa, oviduct, BRL or VERO cells co-cultures, supplemented with 5% or 10% of Fetal Calf Serum (FCS). Cummulus oocyte complexes were aspirated, matured and fertilized in vitro. Embryonic structures were divided into eight treatments. They were placed in culture media TCM 199 containing granulosa, oviduct, BRL or VERO cells, each of them added with 5% or 10% FCS. The conditions for the co-culture were 38.5 ºC, 5% CO2 in air and high humidity for ten consecutive days. Cleavage, blastocyst and hatching rates did not differ (p > 0.05) in co-culture with primary cells (granulosa and oviduct) when FCS concentration increased from 5 to 10%. However, in continuous cells co-culture (BRL and VERO), when FCS concentration increased from 5% to 10%, the blastocyst development rate decreased significantly (p < 0.05) from 33.6 to 16.3% and from 40 to 16.5% in embryo co-culture with BRL and VERO cells, respectively.

Downloads

Download data is not yet available.

Downloads

Published

2011-02-01

How to Cite

Tavares, L. M. T., Feitosa, W. B., Oliveira, V. P. de, Nichi, M., Assumpção, M. E. O. D., & Visintin, J. A. (2011). In vitro development of bovine embryos cultured under different fetal calf serum concentrations and cell types. Brazilian Journal of Veterinary Research and Animal Science, 48(1), 38-45. https://doi.org/10.11606/S1413-95962011000100005

Issue

Section

UNDEFINIED

Most read articles by the same author(s)

<< < 1 2 3 > >>