Holstein and Simmental fresh embryos transfer efficacy

Authors

  • Alicio Martins Junior Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Oziel Bizutti Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Douglas Brasil Nogueira Gaeti Congelamento e Transferência de Embriões Ltda.
  • Renato Campanarut Barnabe Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.1991.51929

Keywords:

Embryo transfer, Superovulation, Fertility of bovine, Blastocyst

Abstract

The efficacy of transferred fresh embryos was evaluated. Donors were superovulated on day 10 of the estrous cycle with 8, 7, 5 e 4 mg (48 mg) or 7, 6,4 e 3 mg (40 mg) of follicle stimulating hormonepituitary (FSH-P) twice a day, during four days. Analogue prostaglandin (cloprostenol) was injected on day 12 of the cycle and inseminations with frozen semen two or three times were performed, each 12 hours. Nonsurgical recovery of the embryos was performed with "Neustad a.d. Aisch" catheter, introducing the medium into the uterine horns with syringes. The uterine horns were flushed with modified Dulbecco's phosphate buffered saline (PBS) enriched with 1% of foetal calf serum at 36 °C. After the recovery, the embryos were morphologically evaluated under a stereomicroscope at 16x and 70x magnifications. The embryos transfers were surgically conducted through flank incision and pregnancy diagnosis made through rectal palpation on day 45-60after transfer. Two hundred twenty one embryos from Holstein cows and thirty seven from Simmental were transferred to two hundred fifty eight cross bred heifers. Pregnancy rates for fresh embryo transfer from Holstein and Simmental cows were 73.3% and 83.3%the values were not statistically different.

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Published

1991-06-01

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Section

NOTES AND INFORMATION

How to Cite

1.
Martins Junior A, Bizutti O, Gaeti DBN, Barnabe RC. Holstein and Simmental fresh embryos transfer efficacy. Braz. J. Vet. Res. Anim. Sci. [Internet]. 1991 Jun. 1 [cited 2024 Apr. 22];28(1):89-92. Available from: https://www.revistas.usp.br/bjvras/article/view/51929