Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns

Authors

  • Liliam Mara Trerrisan Tavares Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Mayra Elena Ortiz D'’Avila Assumpção Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Fernando Ferreira Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Alecsandra Sobreira Lima Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • Marco Roberto Bourg Mello Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP
  • José Antonio Visintin Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Reprodução Animal, São Paulo, SP

DOI:

https://doi.org/10.1590/S1413-95961999000600006

Keywords:

Cells cultured, Epithelial cells, Embryos, Mice

Abstract

Mouse embryos were cultured and co-cultured in mouse (MOEC) and bovine (BOEC) oviduct epithelial cells suspension. Embryos were co-cultured from one cell and two-cell stage to blastocyst stage. Cell culture (MOEC and BOEC) was made at a 100 µl drop under oil. Embryos were recovered 24-26 hours in the case of zygotes and 46-48 hours in the case of 2 cell embryos after hCG injection. Total embryos were randomly split into 3 groups: control (cultured), MOEC and BOEC (co-cultured) with CZB, TCM199 and B2 mediums. In CZB group, 44 zygotes (38.26%) from the control achieved blastocyst stage (expanded or hatched), being statistically different (p<=0.05) from MOEC (1.89%) and BOEC (6.72%). Co-culture with zygotes in TCM199 and B2 didnt pass 2 cell block. Two cell embryos cultured in CZB achieved blastocyst stage at a rate of 43.80% for the control, 38.79% for MOEC and 29.60% for BOEC group. Statistical difference (p<=0.05) appears from the control with BOEC groups. In TCM 199 medium, total of blastocyst were 48.15% for the control, 39.08% for MOEC and 48.49% for the BOEC groups, having no statistical difference among groups. This study allowed concluding that zygote block in TCM199 and B2 medium may be related to the presence of glucose. And that mouse embryos development is not species-specific.

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Published

1999-01-01

Issue

Section

VETERINARY MEDICINE

How to Cite

1.
Tavares LMT, Assumpção MEOD, Ferreira F, Lima AS, Mello MRB, Visintin JA. Zygotes and two cells mouse embryos in co-culture of mouse and bovine epithelial cells in CZB glucose free, TCM199 and Ménézo B2 mediuns. Braz. J. Vet. Res. Anim. Sci. [Internet]. 1999 Jan. 1 [cited 2024 May 19];36(6):308-15. Available from: https://www.revistas.usp.br/bjvras/article/view/5727