Effect of different dose of leucine on glycogen reserves from denervated skeletal muscle of rats

Abstract

The aim of this study was to investigate the action of different doses of leucine on the dynamics ofglycogen reserves of denervated skeletal muscle of rats. Wistar rats aged 3 to 4 months, weighingbetween 200 and 300g being kept under controlled conditions Biotery. The animals were divided intoexperimental group called control (C), denervated (D) treated with amino acid leucine in three doses5nm(CL5), 1.25 mM (CL1,25) and 0.30mM(CL0,30), denervated treated with 0.30mM(DL0,30). Supplementation was performed by gavage and denervation was performed by sectioning the sciatic nerve. Theanalysis of the content of glycogen was performed on samples of the soleus(S), gastrocnemius white(GW)and red(GR). The muscle was lower in S glycogen (-33% and -42,8%), both in CL1,25 and CL5 andincreased in a dose CL0,30(+38%), possibly due to act upon this low dose of insulin secretagogue,promoting an increase in the rate of glucose uptake by peripheral tissues (11,2± 0,3%/min C x 14,16 ±0,2%/min CL0,30). The glycogen's GW and GR presented themselves in small groups CL1,25 (-32,6%)and CL5 (-44,8%) compared to C, however, the group CL0,30 no difference was observed in C. Therewas also decreased in group D (26% S; 35% GW; 28,5% GR) if compared to C. Conclusion: High dosesof leucine may compromise the formation of muscle glycogen and 0.30mM was shown to be effectiveeven when the muscle at rest