FROM MOLECULAR BIOLOGY TO MEDICINE: METHODS COMMONLY USED IN PHARMACOGENETICS

Authors

  • Mario Hiroyuki Hirata Faculty of Pharmaceutical Sciences, University of Sao Paulo
  • Vladimir Tavares Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of Sao Paulo
  • Rosario Dominguez Crespo Hirata Faculty of Pharmaceutical Sciences, University of Sao Paulo

DOI:

https://doi.org/10.11606/issn.2176-7262.v39i4p522-534

Keywords:

Pharmacogenetics. Polymerase Chain Reaction. Polymorphism, Single-Stranded Conformational. Polymorphism, Restriction Fragment Length. DNA Arrays. Denaturing Gradient Gel Electrophoresis.

Abstract

In this review, we discussed the procedures for obtaining, handling and storage of biological samples useful in molecular tests. Methods for screening and direct detection of mutations and gene polymorphisms were presented. Principles, strategies, applications and limitations of polymerase chain reaction (PCR) variations, such as denaturing gradient gel electrophoresis (DGGE), single strain conformational polymorphism (SSCP), restriction fragment length polymorphism (RFLP), allele oligonuleotide-specific amplification (PCR-ASO), Real time PCR, DNA arrays and other molecular techniques, were commented.

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Author Biographies

  • Mario Hiroyuki Hirata, Faculty of Pharmaceutical Sciences, University of Sao Paulo
    Docente
  • Vladimir Tavares, Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of Sao Paulo

     

  • Rosario Dominguez Crespo Hirata, Faculty of Pharmaceutical Sciences, University of Sao Paulo
    Docente

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Published

2006-12-30

Issue

Vol. 39 No. 4 (2006)

Section

Capítulos

License

 

 

How to Cite

1.
Hirata MH, Tavares V, Hirata RDC. FROM MOLECULAR BIOLOGY TO MEDICINE: METHODS COMMONLY USED IN PHARMACOGENETICS. Medicina (Ribeirão Preto) [Internet]. 2006 Dec. 30 [cited 2024 May 17];39(4):522-34. Available from: https://www.revistas.usp.br/rmrp/article/view/403