Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000

Authors

  • Eliana De Stefano Instituto Biológico, São Paulo, SP
  • Wanderley Pereira de Araújo Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, São Paulo, SP
  • Estevão de Camargo Passos Instituto Pasteur, São Paulo, SP
  • Edviges Maristela Pituco Instituto Biológico, São Paulo, SP

DOI:

https://doi.org/10.1590/S1413-95962003000100003

Keywords:

Vesicular Stomatitis, Antibodies, Beef cattle

Abstract

In 2000 a total of 1099 serum samples were obtained of adult beef cattle, from 6 herds located at Auriflama, Gastão Vidigal,, Magda, Santo Antônio do Aracanguá, Sebastianópolis do Sul e Turiúba, counties from the region of Araçatuba. Sera were tested for neutralizing antibodies to vesicular stomatitis virus by microtiter neutralization assay, using the virus strain Indiana 1 Costa Rica/72 with 10(6,0) DICT50/50mL. The indicator cells were African green monkey kidney (VERO-CCL 81), purchased from American Type Cell Colection (ATCC). The neutralization titers of each serum were the reciprocal of the highest dilution expressed at log10 that protected 100.00% of the cell monolayer. Sera with values above 1,6 log10 (1/40) were considered positive for Vesicular Stomatitis virus. Twenty-eight (2.50%) out of 1.099 samples were positive for Vesicular Stomatitis virus Indiana 1, with antibodies titers ranging from 1.9 to 2.8. Except in Turiuba, in the others 5 herds there were positive animals. The presente survey showed the presence the Vesicular Stomatitis virus in the herds stimulating the humoral response. More studies are necessary to verify the site of persistence of the virus.

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Published

2003-01-01

Issue

Section

UNDEFINIED

How to Cite

1.
De Stefano E, Araújo WP de, Passos E de C, Pituco EM. Vesicular Stomatitis: Serological Survey in beef cattle from region of Araçatuba, São Paulo State, Brazil in 2000. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2003 Jan. 1 [cited 2024 Oct. 6];40(1):29-35. Available from: https://www.revistas.usp.br/bjvras/article/view/11388