Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion

Authors

  • Suzana Claudia Spinola dos Santos Universidade Federal da Bahia, Instituto de Ciências da Saúde, Programa de Pós-graduação em Processos Interativos dos Órgãos e Sistemas
  • Ludmila Rodrigues Moroz Universidade Federal da Bahia, Hospital de Medicina Veterinária, Laboratório de Patologia Clínica
  • Mariane Melo dos Santos Universidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Imunologia e Biologia Molecular
  • Allan Souza dos Santos Universidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Imunologia e Biologia Molecular
  • Soraya Castro Trindade Universidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Imunologia e Biologia Molecular
  • Roberto Meyer Universidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Imunologia e Biologia Molecular
  • Maria de Fátima Dias Costa Universidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Neuroquímica e Biologia Celular

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2018.122274

Keywords:

Alloantibodies, Canine antibodies, DEA 1, Blood transfusion in dogs

Abstract

The shortage of dog blood donors in veterinary emergencies can lead to blood transfusions between animals whose blood type has not been identified. The antibody profile serves as a warning sign for animals that require a second blood transfusion, which is only advisable from compatible donor dogs. This article focuses on determination of anti-DEA 1 antibodies using the flow cytometry technique in dogs that have undergone a transfusion using DEA 1-positive blood, compared to results obtained from crossmatching. Blood from 18 DEA 1-positive donors ranked according to the chromatographic technique was used to transfuse thirty-three animals with unknown blood types and which demonstrated negative crossmatching to donors. On post-transfusion days 7, 14, 21 and 28, 45% and 27% of the animals tested positive for the anti-DEA 1 antibody, through crossmatching and flow cytometry, respectively. Detecting antibodies using the flow cytometric technique has high specificity and sensitivity, while crossmatching methods are highly sensitive but manifest low specificity. Following blood transfusion, animals that did not present as positive through crossmatching or flow cytometry were considered different from all other DEA 1-positive blood groups.

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Published

2018-04-04

Issue

Section

FULL ARTICLE

How to Cite

1.
Santos SCS dos, Moroz LR, Santos MM dos, Santos AS dos, Trindade SC, Meyer R, et al. Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2018 Apr. 4 [cited 2024 Jul. 17];55(1):1-7. Available from: https://www.revistas.usp.br/bjvras/article/view/122274