Assessment of different in vitro sperm challenges and in vivo fertility of bovine semen batches

Authors

  • Letícia Zoccolaro Oliveira Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Clínica e Cirurgia Veterinárias
  • Lucas Bernardes Ribeiro Centro Universitário de Rio Preto, Faculdade de Medicina Veterinária
  • Luiz Gustavo Teodoro da Silva Centro Universitário de Rio Preto, Faculdade de Medicina Veterinária
  • Clara Slade Oliveira Centro Nacional de Pesquisa em Gado de Leite
  • Erika Aline Ribeiro Dias Instituto de Zootecnia
  • Suzane Peres Campanholi Instituto de Zootecnia
  • Carla Cristian Campos Universidade Federal de Uberlândia, Faculdade de Medicina Veterinária
  • Maiana Visoná de Oliveira Universidade Federal de Uberlândia, Faculdade de Medicina Veterinária
  • Fabio Morato Monteiro Instituto de Zootecnia

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2017.133366

Keywords:

Conception rate, In vitro sperm resistance, Timed-AI

Abstract

The aim of this work was to submit sperm cells to different laboratory challenges and  to compare in vitro results with in vivo semen fertility. Four different batches from the same Brangus bull were used in a timed-AI program of 332 Brangus cows. Each batch (B) was submitted to the following procedure: semen sample was thawed at 36°C for 30 seconds (control). Sperm motility parameters, plasma membrane integrity, sperm morphology, and concentration were assessed. Then, an aliquot of thawed sample was incubated in a water bath at 45°C for 40 min (thermal challenge group; TCG) and another aliquot was centrifuged at 500 xg (Percoll gradient 45%/90%) for 15 min (centrifugation challenge group; CCG). Centrifuged semen was also submitted to another thermal challenge, being incubated (water bath) at 45°C for 40 min (centrifugation + thermal challenge group; CTCG). At the end of each challenge (CCG, TCG, and CTCG), the same laboratory tests used for control group were repeated. The following conception rates (CR) were observed for each batch: B1 = 48.9% (44/90); B2 = 44.2% (23/52); B3 = 55.5% (40/72); B4 = 43.2% (51/118); (p < 0.10). In the lab, B3 presented higher (p ≤ 0.05) progressive motility (PM) than B4 after thawing (control group) and after all sperm challenges (TCG, CCG, and CTCG). However, despite B3 and B4 having demonstrated a similar percentage of plasma membrane integrity (PMI) to the control group (B3 = 66.7 ± 1.3 and B4 = 65.2 ± 3.3), B3 demonstrated higher (P ≤ 0.05) percentage of PMI (37.2 ± 2.5) than B4 (26.7 ± 3.3) after passing through the most stressing in vitro challenge (CTCG). The semen batch presenting the highest resistance to in vitro challenges was the one that presented a trend for higher in vivo fertility, suggesting that submitting semen samples to laboratory challenges may be an interesting alternative for selecting batches with greater field fertility.

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Author Biography

Letícia Zoccolaro Oliveira, Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Clínica e Cirurgia Veterinárias

Departamento de Clínica e Cirurgia Veterinárias (setor de Reprodução Animal)

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Published

2017-11-24

How to Cite

Oliveira, L. Z., Ribeiro, L. B., Silva, L. G. T. da, Oliveira, C. S., Dias, E. A. R., Campanholi, S. P., Campos, C. C., Oliveira, M. V. de, & Monteiro, F. M. (2017). Assessment of different in vitro sperm challenges and in vivo fertility of bovine semen batches. Brazilian Journal of Veterinary Research and Animal Science, 54(3), 264-276. https://doi.org/10.11606/issn.1678-4456.bjvras.2017.133366

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