Frequency of dog erythrocyte antigen 1 blood group and risk of incompatible transfusion in dogs of different breeds and mongrels from the city of Salvador - BA, Brazil

Authors

  • Suzana Cláudia Spínola dos Santos Universidade Federal da Bahia, Instituto de Ciências da Saúde, Programa de Pós-Graduação em Processos Interativos dos Órgãos e Sistemas, Salvador - BA, Brasiil http://orcid.org/0000-0001-5852-3689
  • Mariane Melo dos Santos Universidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Imunologia e Biologia Molecular, Salvador - BA, Brasil http://orcid.org/0000-0003-3561-8938
  • Wellington Francisco Rodrigues Universidade Federal do Triângulo Mineiro, Programa de Pós-graduação em Ciências da Saúde/Biologia Celular, Uberaba - MG, Brasil https://orcid.org/0000-0002-3426-2186
  • Roberto Meyer Universidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Imunologia e Biologia Molecular, Salvador - BA, Brasil https://orcid.org/0000-0002-4727-4805
  • Maria de Fátima Dias Costa Universidade Federal da Bahia, Instituto de Ciências da Saúde, Laboratório de Neuroquímica e Biologia Celular, Salvador - BA, Brasil https://orcid.org/0000-0003-0916-2893

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2019.154865

Keywords:

DEA 1, Typification in dogs, Transfusion risk in dogs, Veterinary transfusion medicine

Abstract

The dog erythrocyte antigen 1 (DEA 1) is the most immunogenic blood group in dogs, and blood transfusions may trigger some undesirable effects in veterinary patients, which are directly associated with incompatible transfusions. The present study aimed to investigate the frequency of positive DEA 1 blood group in blood donor dogs from a blood bank in Salvador, Bahia, Brazil, and also to calculate the risk of managing incompatible blood in both first and second transfusion. A number of 203 dogs of different breeds, aged between 1 and 8 years, weighing 28 kg, with no degree of kinship and of both sexes in Salvador - BA, Brazil were evaluated to investigate the blood type DEA 1 frequency, by means of chromatography and flow cytometry tests for blood typing. The risk of incompatible blood transfusion in either a first or a second transfusion was also calculated. The frequency of the DEA 1 group ranged from 0% to 100% in various breeds, but with a mean positivity of 62.07% (126/203). And the lowest risk of an DEA 1 negative animal receiving DEA 1 positive blood within the group of animals evaluated was 0.92% at a first transfusion; and the risk of the same animal receiving incompatible blood for the DEA group 1 in the second transfusion was 0.008%. The highest risk of an DEA 1 negative animal receiving DEA 1 positive blood from these animals was 69.12%; and the risk of receiving incompatible blood for DEA 1 was 47.77%. In conclusion, the frequency of the DEA 1 group varied between the studied breeds and the risk of incompatible blood transfusions varies according to donor and recipiente breeds, but this can be overridden if blood typing tests are performed along with the cross-reaction test for compatibility.

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Author Biography

Suzana Cláudia Spínola dos Santos, Universidade Federal da Bahia, Instituto de Ciências da Saúde, Programa de Pós-Graduação em Processos Interativos dos Órgãos e Sistemas, Salvador - BA, Brasiil

Universidade Federal da Bahia, Instituto de Ciências da Saúde, Programa de Pós-graduação em Processos Interativos dos Órgãos e Sistemas, Salvador – BA, Brazil

Doutoranda

References

Acierno MM, Raj K, Giger U. DEA 1 expression on dog erythrocytes analysed by immunochromatographic and flow cytometric techniques. J Vet Intern Med. 2014;28(2):592- 8. http://dx.doi.org/10.1111/jvim.12321. PMid:24611973.

Arango HG. Bioestatistica teórica e computacional. 2a ed. Rio de Janeiro: Guanabara Koogan; 2005. 423 p.

Carli E, Carminato A, Ravagnan S, Capello K, Antognoni MT, Miglio A, Furlanello T, Proverbio D, Spada E, Stefani A, Mutinelli F, Vascellari M. Frequency of DEA 1 antigen in 1037 mongrel and purebred dogs in Italy. BMC Vet Res. 2017;13:364. http://dx.doi.org/10.1186/s12917-017-1286-y. PMid:29187176.

Dhliwayo S, Makonese TA, Whittall B, Chikerema SM, Pfukenyi DM, Tivapasi MT. A study on the prevalence of dog erythrocyte antigen 1.1 and detection of canine Babesia by polymerase chain reaction from apparently healthy dogs in a selected rural community in Zimbabwe. J S Afr Vet Assoc. 2016;87(1):1-5. http://dx.doi.org/10.4102/jsava. v87i1.1409. PMid:27796108.

Ekiz EE, Arslan M, Ozcan M, Gultekin GI, Gulay OY, Kirmizibayrak T, Giger U. Frequency of dog erythrocyte antigen 1.1 in 4 breeds native to different areas in Turkey. Vet Clin Pathol. 2011;40(4):518-23. http://dx.doi.org/10.1111/ j.1939-165X.2011.00370.x. PMid:22136478.

Esteves VS, Lacerda LA, Lasta CS, Pedralli V, González FHD. Frequencies of DEA blood types in a purebreed canine blood donor population in Porto Alegre, R.S., Brazil. Pesq Vet Bras. 2011;31(2):178-81. http://dx.doi.org/10.1590/ S0100-736X2011000200015.

Ferreira RRF, Gopegui RR, Matos AJF. Frequency of dog erythrocyte antigen 1.1 expression in dogs from Portugal. Vet Clin Pathol. 2011;40(2):198-201. http://dx.doi.org/10.1111/j.1939-165X.2011.00311.x. PMid:21554361.

Giger U. Blood typing and crossmatching to ensure blood compatibility. In: Bonagura JD, Twedt DC, editors. Kirk’s current veterinary therapy XV. Philadelphia: Saunders; 2014. p. 143-8. Giger U, Stieger K, Palos H. Comparison of various canine blood-typing methods. Am J Vet Res. 2005;66(8):1386- 92. http://dx.doi.org/10.2460/ajvr.2005.66.1386. PMid:16173482.

Gracner D, Bedrica L, Potocnjak D, Sakar D, Samardzija M, Capak H, Gracner GG. Blood groups and haematology indicators in Croatian indigenous breeds of dog. II Dalmatian dog. Vet Arh [Internet]. 2011 [cited 2017 Jan 20];81(1):111-7. Available from: http://www.vef.unizg.hr/ vetarhiv/papers/2011-81-1-11.pdf

Hohenhaus AE. Importance of blood groups and blood group antibodies in companion animals. Transfus Med Rev. 2004;18(2):117-26. http://dx.doi.org/10.1016/j. tmrv.2003.12.003. PMid:15067591.

Iazbik MC, O’Donnell M, Marin L, Zaldivar S, Hudson D, Couto CG. Prevalence of dog erythrocyte antigens in retired racing greyhounds. Vet Clin Pathol. 2010;39(4):433- 5. http://dx.doi.org/10.1111/j.1939-165X.2010.00261.x. PMid:21198732.

Mesa-Sanchez I, Ruiz de Gopegui-Fernández R, GranadosMachuca MM, Galan-Rodriguez A. Prevalence of dog erythrocyte antigen 1.1 in galgos (Spanish greyhounds). Vet Rec. 2014;174:351. http://dx.doi.org/10.1136/vr.102087. PMid:24578316.

Milczak A, Abramowicz B, Madany J, Winiarczyk D, Wrzesniewska K, Bochynska D, Sahinduran S. Frequency of DEA 1.1 antigen in german shepherds. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies. 2016;18(3):221-4. http:// dx.doi.org/10.15421/nvlvet7151.

Novais AA, Santana AE, Vincentin LA. Prevalência do grupo sanguíneo DEA 1 (subgrupos 1.1 e 1.2) em cães (canis familiaris, Linaeus, 1758) criados no Brasil. Braz J Vet Res Anim Sci.. 1999;36(1):23-7. http://dx.doi.org/10.1590/ S1413-95961999000100004.

Riond B, Schuler E, Rogg E, Hofmann-Lehmann R, Lutz H. Prevalence of dog erythrocyte antigen 1.1 in dogs in Switzerland evaluated with the gel columm technique. Schweiz Arch Tierheilkd. 2011;153(8):369-74. http://dx.doi. org/10.1024/0036-7281/a000223. PMid:21780065.

Santos SCS, Moroz LR, Santos MM, Santos AS, Trindade SC, Meyer R, Costa MFD. Detection of canine anti-DEA 1 antibodies using flow cytometry in dogs following DEA 1-positive blood transfusion. Braz J Vet Res Anim Sci. 2018;55(1):1-7. http://dx.doi.org/10.11606/issn.1678-4456. bjvras.2018.122274.

Souza SL, Stopiglia AJ, Gomes SGR, Ulata SK, Moroz LR, Fantoni DT. Estudo da frequência dos antígenos eritrocitários caninos 1, 1.1 e 7 e risco de transfusão incompatível em cães de diferentes raças e mestiços da região metropolitana da cidade de São Paulo-SP, Brasil. Braz J Vet Res Anim Sci. 2014;51(4):316-23. http://dx.doi.org/10.11606/issn.1678- 4456.v51i4p316-323.

Spada E, Proverbio D, Flórez LMV, Chamizo MRP, Perego R, De Giorgi GB, Baggiani L. Prevalence of dog erythrocyte antigens 1, 4 and 7 in galgos (Spanish Greyhounds). J Vet Diagn Invest. 2015;27(4):558-61. http://dx.doi. org/10.1177/1040638715592025. PMid:26179093.

Spada E, Proverbio D, Flórez LMV, Serna BSG, Chamizo MRP, Baggiani L, Perego R. Prevalence of dog erythrocyte antigens 1, 4 and 7 in Podenco Ibicenco (Ibizan Hounds) from Ibiza Island. Vet Med Int. 2016;2016:1048257. http:// dx.doi.org/10.1155/2016/1048257. PMid:27034890.

Tocci LJ, Ewing PJ. Increasing patient safety in veterinary transfusion medicine: an overview of pre transfusion testing. J Vet Emerg Crit Care. 2009;19(1):66-73. http://dx.doi. org/10.1111/j.1476-4431.2009.00387.x. PMid:19691586.

Zubcic D, Bedrica L, Gracner D, Harapin I, Fury M, Jeremic J. Blood groups, haematology and clinicolchemical indicators in indigenous breeds of dog. I. Croatian sheepdog. Vet Arh [Internet]. 2008 [cited 2015 Feb 18];78(2):141-7. Available from: http://www.vef.unizg.hr/vetarhiv/papers/2011-81-1-11.pdf

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Published

2019-10-25

How to Cite

Santos, S. C. S. dos, Santos, M. M. dos, Rodrigues, W. F., Meyer, R., & Costa, M. de F. D. (2019). Frequency of dog erythrocyte antigen 1 blood group and risk of incompatible transfusion in dogs of different breeds and mongrels from the city of Salvador - BA, Brazil. Brazilian Journal of Veterinary Research and Animal Science, 56(3), e154865. https://doi.org/10.11606/issn.1678-4456.bjvras.2019.154865

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