Factors associated with the efficiency of acaricides on different populations of Rhipicephalus microplus


  • Michel Menin Universidade do Oeste de Santa Catarina
  • Carolain Xavier Universidade do Oeste de Santa Catarina
  • Mauricio Francisco Grigolo Universidade do Oeste de Santa Catarina
  • Kaio Fernando Molosse Universidade do Oeste de Santa Catarina
  • Michele Helena Weirich Universidade do Oeste de Santa Catarina
  • Bruna Matzembacker Universidade do Oeste de Santa Catarina
  • Silvana Giacomini Collet Universidade do Oeste de Santa Catarina
  • Alan Miranda Prestes Universidade do Oeste de Santa Catarina
  • Giovana Camillo Universidade do Oeste de Santa Catarina




Tick, In vitro, Acaricides, Control, Resistance


Tick infestation causes major problems in cattle. Tick parasitism accounts for significant economic losses in many beef and dairy herds in the vast majority of the states in the Brazilian territory including the State of Santa Catarina in the southern region of the country. Tick resistance to several active principles occurs due to a number of factors including the indiscriminate, injudicious and inadequate use of tick insecticides. Considering the great importance of fighting off tick infestations in cattle, we evaluated the efficiency of 8 different topical active principles against the ixodid tick R. microplus and identified the main factors that contribute to the development of ticks that are resistant to acaricides in the farms and bovine herds studied. For such purpose, R. microplus telegionae were collected in 39 farms locates in different municipalities of the West of Santa Catarina, south Brazil. At the time of sample collection, information about the management, the history of the acar used in the herd, the number of annual applications of these pesticides, the frequency of acaricide rotation, and the frequency of technical monitoring for strategic tick control were retrieved. We collected this data in order determine contributing factors to the development resistant R. microplus populations. For the sensitivity profile of these ticks to a number of different acaricides tested, 10 engorged females for each principle and a control were used. The results of our study show that most associations between pyrethroids and organophosphates had an efficiency between 96.6% and 100% in the control of R. microplus except for the combination of Alfacipermetrine 15%, Ethion 16%, and Chlorpyrifos 8.5% that had 93.4% of efficiency. Among the chemicals not associated with any other type of compound, Amitraz 12.5% ​​was effective in 98.3% of the cases. In contrast, Cypermethrin 15% had an efficiency in only 25.8% of the farms/herds analyzed. Based on the results of the present study, we may infer that the methods of control and management used by producers may be related to the low indexes of resistance to topical acaricides in the different populations of ticks in the study area.


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Amaral MAZ, Rocha CM, Faccini JL, Furlong J, Monteiro CM, Prata MC. Strategic control of cattle ticks: milk producers’ perceptions. Rev Bras Parasitol Vet. 2011;20(2):148-54. http://dx.doi.org/10.1590/S1984-29612011000200010. PMid:21722490.

Brasil. Ministério da Agricultura. Normas para registros de parasiticidas de uso pecuário no Brasil. Brasília: Ministério da Agricultura; 1997.

Brito LG, Silva Netto FG, Oliveira MCS, Barbieri FS. Bio-ecologia, importância médico-veterinária e controle de carrapatos, com ênfase no carrapato de bovinos, Rhipicephalus (Boophilus) microplus [Internet]. Porto Velho: Embrapa; 2006 [cited 2019 Jan 20] 21 p. (Documentos; 104). Available from: https://www.embrapa. br/busca-de-publicacoes/-/publicacao/710607/bio-ecologiaimportancia-medicoveterinaria-e-controle-de-carrapatoscom-enfase-no-carrapato-dos-bovinos-rhipicephalusboophilus-microplus

Camillo G, Vogel FF, Sangioni LA, Cadore GC, Ferrari R. In vitro evaluation of acaricides efficiency to bovine’s ticks of Rio Grande do Sul State, Brazil. Cienc Rural. 2009;39(2):490- 5. http://dx.doi.org/10.1590/S0103-84782008005000082.

Campos DA Jr, Oliveira PR. In vitro valuation of acaricides efficiency to Boophilus microplus (Canestrini, 1887) (Acari: Ixodidae) from bovines at the region of Ilhéus, Bahia, Brazil. Cienc Rural. 2005;35(6):1386-92. http://dx.doi.org/10.1590/ S0103-84782005000600025.

Cody R. Uma introdução à edição universitária do SAS. São Paulo: Instituto SAS; 2018. Drummond RO, Ernst SE, Trevino JL, Gladney WJ, Graham OH. Boophilus annulatus and Boophilus microplus: laboratory tests for insecticides. J Econ Entomol. 1973;66(1):130-3. http://dx.doi.org/10.1093/jee/66.1.130. PMid:4690254.

Fernández-Salas A, Rodríguez-Vivas RI, Alonso-Díaz MÁ. Resistance of Rhipicephalus microplus to Amitraz and Cypermethrin in Tropical Cattle Farms in Veracruz, Mexico. J Parasitol. 2012;98(5):1010-4. http://dx.doi.org/10.1645/ GE-3074.1. PMid:22524292.

Furlong J, Martins JR, Prata MCA. O carrapato dos bovinos e a resistência: temos o que comemorar? Hora Vet. [Internet]. 2007 [cited 2019 Jan 20];27(159):1-7. Available from: http:// r1.ufrrj.br/adivaldofonseca/wp-content/uploads/2014/06/ Artigo-A-Hora-Veterinária-Set-07.pdf

Furlong J, Sales RO. Strategical control of cattle tick in the Milk Bovine: a revision. Rev Bras Hig Sanid Anim. 2007;1(2):44- 72. http://dx.doi.org/10.5935/1981-2965.20070009.

Gomes A, Koller WW, Barros ATM. Susceptibility of Rhipicephalus (Boophilus) microplus to acaricides in Mato Grosso do Sul, Brazil. Cienc Rural. 2011;41(8):1447-52. http://dx.doi.org/10.1590/S0103-84782011005000105.

Grisi L, Leite RC, Martins JR, Barros AT, Andreotti R, Cançado PH, León AA, Pereira JB, Villela HS. Reassessment of the potential economic impact of cattle parasites in Brazil. Braz. J. Vet. Parasitol. 2014;23(2):150-6. http:// dx.doi.org/10.1590/S1984-29612014042. PMid:25054492.

IBGE: Instituto Brasileiro de Geografia e Estatística [Internet]. Produção da pecuária municipal. Vol. 44. Rio de Janeiro: IBGE; 2016 [cited 2019 Jan 20]. Available from: https://biblioteca.ibge.gov.br/visualizacao/periodicos/84/ ppm_2016_v44_br.pdf

Jonsson NN. The productivity effects of cattle tick (Boophilus microplus) infestation on cattle, with particular reference to Bos indicus cattle and their crosses. Vet Parasitol. 2006;137(1- 2):1-10. http://dx.doi.org/10.1016/j.vetpar.2006.01.010. PMid:16472920.

Machado FA, Pivoto FL, Ferreira MS, Gregorio FV, Vogel FS, Sangioni LA. Rhipicephalus (Boophilus) microplus in the western-central region of Rio Grande do Sul, Brazil: multiresistant tick. Rev Bras Parasitol Vet. 2012;23(3):337- 42. http://dx.doi.org/10.1590/S1984-29612014063. PMid:25271453.

Pazinato R, Klauck V, Grosskopf RK, Dalla Rosa L, Volpato A, Baretta D, Stefani LM, Silva AS. Antiparasitic resistance of different populations of ticks (Rhipicephalus microplus) in the Western of Santa Catarina State, Brazil. Acta Scientiae Veterinariae [Internet]. 2014 [cited 2019 Jan 20];42(1):1-6. Available from: https://www.researchgate. net/publication/279022655_Antiparasitic_Resistance_of_ Different_Populations_of_ticks_Rhipicephalus_microplus_ in_the_Western_of_Santa_Catarina_State_Brazil

Reck J, Marks FS, Rodrigues RO, Souza UA, Webster A, Leite RC, Gonzales JC, Klafke GM, Martins JR. Does Rhipicephalus microplus tick infestation increase the risk for myiasis caused by Cochliomyia hominivorax in cattle? Prev Vet Med. 2014;113(1):59-62. http://dx.doi.org/10.1016/j. prevetmed.2013.10.006. PMid:24176137.

Reginato C, Cadore GC, Menezes FR, Sangioni LA, Vogel FSF. Efficacy of commercial synthetic pyrethroids and organophosphates associations used to control Rhipicephalus (Boophilus) microplus in Southern Brazil. Rev Bras Parasitol Vet. 2017;26(4):500-5004. http://dx.doi.org/10.1590/s1984- 29612017054. PMid:29091122.

Rocha CMBM, Leite RC, Bruhn FRP, Guimarães AM, Furlong J. Perceptions of milk producers from Divinópolis, Minas Gerais, regarding Rhipicephalus (Boophilus) microplus control. Rev Bras Parasitol Vet. 2011;20(4):295-302. http://dx.doi. org/10.1590/S1984-29612011000400007. PMid:22166383.

Santos FCC, Vogel FSF. Amitraz and cypermethrin resistance ticks Rhipicephalus (Boophilus) microplus in cattle herds located in Rio Grande do Sul from 2005 to 2011. Rev Port Ciênc Vet. [Internet]. 2012 [cited 2019 Jan 20];107(581- 582):121-124. Available from: http://www.fmv.ulisboa.pt/ spcv/PDF/pdf6_2012/121-124.pdf

Veríssimo CJ. Resistência e controle do carrapato-do-boi. Nova Odessa: Instituto de Zootecnia; 2015. 144 p. Willadesen P, Jongejan F. Immunology of the tick-host interaction and the control of tick-borne diseases. Parasitol Today. 1999;15(7):258-562. http://dx.doi.org/10.1016/ S0169-4758(99)01472-6. PMid:10377526.




How to Cite

Menin, M., Xavier, C., Grigolo, M. F., Molosse, K. F., Weirich, M. H., Matzembacker, B., Collet, S. G., Prestes, A. M., & Camillo, G. (2019). Factors associated with the efficiency of acaricides on different populations of Rhipicephalus microplus. Brazilian Journal of Veterinary Research and Animal Science, 56(3), e157595. https://doi.org/10.11606/issn.1678-4456.bjvras.2019.157595