Serological survey of Rickettsia in equids from Vale do Paraíba, São Paulo, Brazil, and their tick identification and molecular investigation of Rickettsia

Authors

  • Claudia Iorio Budweg Universidade Santo Amaro, Mestrado em Medicina e Bem-Estar Animal, São Paulo – SP, Brazil
  • Amanda Oliveira de Sousa Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo – SP, Brazil
  • Tânia Regina Vieira de Carvalho Universidade Santo Amaro, Mestrado em Medicina e Bem-Estar Animal, São Paulo – SP, Brazil
  • Zahi Êni Santos Souza Universidade Santo Amaro, Mestrado em Medicina e Bem-Estar Animal, São Paulo – SP, Brazil
  • Maria Carolina de Azevedo Serpa Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo – SP, Brazil
  • Thiago Fernandes Martins Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo – SP, Brazil
  • Fernanda Nieri-Bastos Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo – SP, Brazil
  • Arlei Marcili Universidade Santo Amaro, Mestrado em Medicina e Bem-Estar Animal, São Paulo – SP, Brazil; Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo – SP, Brazil
  • Marcelo Bahia Labruna Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo – SP, Brazil
  • Jonas Moraes-Filho Universidade Santo Amaro, Mestrado em Medicina e Bem-Estar Animal, São Paulo – SP, Brazil; Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo – SP, Brazil

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2019.158159

Keywords:

Ticks, Amblyomma, Dermacentor, qPCR, Host

Abstract

Brazilian spotted fever is a serious and lethal illness for humans and is caused by the Rickettsia rickettsii bacteria. In the state of São Paulo/SP (Brazil), the etiological agent of this disease is transmitted by the Amblyomma sculptum tick. It was already shown that horses infected with this bacteria produce a strong immune response and could be important sentinels for the detection of the disease in a proper region. The present investigation performed a serological survey in horses from five farms of Vale do Paraíba, São Paulo state, Brazil, searching for antibodies against, Rickettsia rickettsii, Rickettsia parkeri, Rickettsia amblyommatis, Rickettsia rhipicephali, and Rickettsia bellii. In each farm, ticks were also collected that were taxonomically identified and examined by real-time PCR for Rickettsia spp DNA. Blood samples were collected from 206 horses, and 334 ticks were picked up from these animals from January to December 2017. Eighty ticks were
A. sculptum and 254 Dermacentor nitens. Of the blood samples, 7.3% seroconverted to Rickettsia spp. Of these, 0.97% had a positive serological response to R. bellii. None of the 80 A. sculptum ticks were positive through real-time PCR for Rickettsia spp. Although there was no detection of ticks infected by Rickettsia spp in five farms of Paraíba Valley, the horses presented serological positive reactions against this agent. Thus, further large studies should be conducted in the area targeting hosts and vectors to generate data for control measures of the transmission of Brazilian spotted fever. 

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References

Aragão HB. Ixodidas brasileiros e de alguns paizes limítrofes. Mem Inst Oswaldo Cruz. 1936;31(4):759-843. http://dx.doi. org/10.1590/S0074-02761936000400004.

Barros-Battesti DM, Arzua M, Bechara GH. Carrapatos de importância médico-veterinária da região neotropical: um guia ilustrado para identificação de espécies. São Paulo: Vox/ICTTD-3/Butantan; 2006.

Batista FG, Silval DM, Green KT, Tezza LBL, Vasconcelos SP, Carvalho SGS, Silveira I, Moraes-Filho J, Labruna MB, Fortes FS, Molento MB. Serological survey of Rickettsia sp. in horses and dogs in an non-endemic area in Brazil. Rev Bras Parasitol Vet. 2010;19(4):205-9.

Cardoso LD, Freitas RN, Mafra CL, Neves CVB, Figueira FCB, Labruna MB, Gennari SM, Walker DH, Galvão MAM. Caracterização de Rickettsia spp. circulante em foco silencioso de febre maculosa brasileira no Município de Caratinga, Minas Gerais, Brasil. Cad Saúde Pública, 2006;22(3):495-501.

CVE: Centro de Vigilância Epidemiológica Prof. “Alexandre Vranjac”. Febre maculosa brasileira. São Paulo: CVE; 2019. Informe Técnico II.

Freitas MC, Grycajuk M, Molento MB, Bonacin J, Labruna MB, Pacheco RC, Moraes-Filho J, Deconto I, Biondo AW. Brazilian spotted fever in cart horses in a non-endemic area in Southern Brazil. Rev Bras Parasitol Vet. 2010;19(2):130- 1. http://dx.doi.org/10.1590/S1984-29612010000200013. PMid:20624353.

Guedes E, Leite RC, Prata MCA, Pacheco RC, Walker DH, Labruna MB. Detection of Rickettsia rickettsii in the tick Amblyomma cajennense in a new Brazilian spotted fever-endemic area in the state of Minas Gerais. Mem Inst Oswaldo Cruz. 2005;100(8):841-5. http://dx.doi.org/10.1590/ S0074-02762005000800004. PMid:16444414.

Horta MC, Vianna MCB, Mafra CL, Schumaker TTS, Walker DH, Galvão MAM, Sangioni LA, Vidotto O, Gennari SM, Labruna MB. Prevalence of antibodies to spotted fever group rickettsiae in humans and domestic animals in a Brazilian spotted fever-endemic area in the state of São Paulo, Brazil: serologic evidence for infection by Rickettsia rickettsii and another spotted fever group Rickettsia. Am J Trop Med Hyg. 2004;71(1):93-7. http://dx.doi.org/10.4269/ ajtmh.2004.71.93. PMid:15238696.

Krawczak FS, Labruna MB, Hecht JA, Paddock CD, Karpathy SE. Genotypic characterization of Rickettsia bellii reveals distinct lineages in the United States and South America. BioMed Res Int. 2018;2018:1-8. http://dx.doi. org/10.1155/2018/8505483. PMid:29850579.

Labruna MB, Kasai N, Ferreira F, Faccini JLH, Gennari SM. Seasonal dynamics of ticks (Acari: Ixodidae) on horses in the state of São Paulo, Brazil. Vet Parasitol. 2002;105(1):65- 77. http://dx.doi.org/10.1016/S0304-4017(01)00649-5. PMid:11879967.

Labruna MB, Ogrzewalska M, Soares JF, Martins TF, Soares HS, Moraes-Filho J, Nieri-Bastos FA, Almeida AP, Pinter A. Experimental Infection of Amblyomma aureolatum Ticks with rickettsia rickettsii. Emerg Infect Dis. 2011;17(5):829-34. http://dx.doi.org/10.3201/eid1705.101524. PMid:21529391.

Labruna MB, Pacheco RC, Richtzenhain LJ, Szabó MP. Isolation of Rickettsia rhipicephali and Rickettsia bellii from Haemaphysalis juxtakochi Ticks in the State of São Paulo, Brazil. Appl Environ Microbiol. 2007;73(3):869-73. http://dx.doi.org/10.1128/AEM.02249-06. PMid:17142361.

Labruna MB, Whitworth T, Horta MC, Bouyer DH, Mcbride JW, Pinter A, Popov V, Gennari SM, Walker DH. Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of São Paulo, Brazil, where Brazilian spotted fever is endemic. J Clin Microbiol. 2004;42(1):90-8. http:// dx.doi.org/10.1128/JCM.42.1.90-98.2004. PMid:14715737.

Lemos-Monteiro J, Fonseca F, Prado A. Typho exantemático de São Paulo. Novas experiências sobre a transmissão experimental por carrapatos. Bras Med. 1932;16(48):993-5.

Martinelli M. Clima do estado de São Paulo. Revista FrancoBrasileira de Geografia. 2010;8(8):20-32.

Medeiros AP, Moura AB, Souza AP, Bellato V, Sartor AA, Vieira-Neto A, Moraes-Filho J, Labruna MB. Antibodies against rickettsiae from spotted fever groups in horses from two mesoregions in the state of Santa Catarina, Brazil. Arq Bras Med Vet Zootec. 2013;65(6):1713-9. http://dx.doi. org/10.1590/S0102-09352013000600019.

Murray GGR, Weinert LA, Rhule EL, Welch JJ. The Phylogeny of Rickettsia using different evolutionary signatures: how tree-like is bacterial evolution? Syst Biol. 2016;65(2):265-79.

Pacheco RC, Moraes–Filho J, Guedes E, Silveira I, Richtzenhain LJ, Leite RC, Labruna MB. Rickettsial infections of dogs, horses and ticks in Juiz de Fora, southeastern Brazil, and isolation of Rickettsia rickettsii from Rhipicephalus sanguineus ticks. Med Vet Entomol. 2011;25(2):148-55. http://dx.doi. org/10.1111/j.1365-2915.2010.00915.x. PMid:20950356.

Pinter A, Labruna MB. Isolation of Rickettsia rickettsii and Rickettsia bellii in cell culture from the tick Amblyomma aureolatum in Brazil. Ann N Y Acad Sci. 2006;1078(1):523-9. http://dx.doi.org/10.1196/annals.1374.103. PMid:17114770.

Piza JT. O carrapato como transmissor do Typho exanthemático de São Paulo? Boletim da Sociedade e Cirúrgica de São Paulo. 1932;15(2):350. Ricketts HT. Some aspects of Rocky Montain Spotted, Fever as shown by recent investigations. Med Rec. 1909;(76):843-55.

Sangioni LA, Horta MC, Vianna MCB, Gennari SM, Soares RM, Galvão MAM, Schumaker TTS, Ferreira F, Vidotto O, Labruna MB. Rickettsial infection in animals and Brazilian Spotted Fever endemicity. Emerg Infect Dis. 2005;11(2):265-70. http://dx.doi.org/10.3201/eid1102.040656. PMid:15752445.

Silveira I, Pacheco RC, Szabó MPJ, Ramos HGC, Labruna MB. Rickettsia parkeri in Brazil. Emerg Infect Dis. 2007;13(7):1111-3. http://dx.doi.org/10.3201/eid1307.061397. PMid:18214195.

Toledo RS, Tamekuni K, Silva M Fo, Haydu VB, Barbieri AR, Hiltel AC, Pacheco RC, Labruna MB, Dumler JS, Vidotto O. Infection by spotted fever rickettsiae in people, dogs, horses and ticks in Londrina, Parana State, Brazil. Zoonoses Public Health. 2009;58(6):416-23. http://dx.doi. org/10.1111/j.1863-2378.2010.01382.x. PMid:21824336.

Ueno TE, Costa FB, Moraes-Filho J, Agostinho WC, Fernandes WR, Labruna MB. Experimental infection of horses with Rickettsia rickettsii. Parasit Vectors. 2016;9(1):499. http:// dx.doi.org/10.1186/s13071-016-1784-y. PMid:27624315.

Vieira AML, Souza CE, Labruna MB, Mayo RC, Souza SSL, Camargo-Neves VLF. Manual de vigilância acarológica - estado de São Paulo. São Paulo: SUCEN; 2004. p. 62.

Weinert LA, Werren JH, Aebi A, Stone GN, Jiggins FM. Evolution and diversity of Rickettsia bacteria. BMC Biol. 2009;7(1):1-15. http://dx.doi.org/10.1186/1741-7007-7-6. PMid:19187530.

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Published

2019-12-09

How to Cite

Budweg, C. I., Sousa, A. O. de, Carvalho, T. R. V. de, Souza, Z. Êni S., Serpa, M. C. de A., Martins, T. F., Nieri-Bastos, F., Marcili, A., Labruna, M. B., & Moraes-Filho, J. (2019). Serological survey of Rickettsia in equids from Vale do Paraíba, São Paulo, Brazil, and their tick identification and molecular investigation of Rickettsia. Brazilian Journal of Veterinary Research and Animal Science, 56(4), e158159. https://doi.org/10.11606/issn.1678-4456.bjvras.2019.158159

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