In vitro evaluation of the dissolving effect of carbonated beverages (Coca-Cola® ) and enzyme-based solutions on enteroliths obtained from horses: pilot study

Authors

  • Sergio Andrés Vélez Gil University of Antioquia, Faculty of Agrarian Sciences, School of Veterinary Medicine, Centauro Research Group, Research Line in Equine Medicine and Surgery
  • Juan José Patiño Marulanda University of Antioquia, Faculty of Agrarian Sciences, School of Veterinary Medicine, Centauro Research Group, Research Line in Equine Medicine and Surgery
  • José Ramón Martínez Aranzales University of Antioquia, Faculty of Agrarian Sciences, School of Veterinary Medicine, Centauro Research Group, Research Line in Equine Medicine and Surgery https://orcid.org/0000-0003-2428-1372

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2021.182579

Keywords:

Horse, Colic, Enterolithiasis, Intestine, Obstruction

Abstract

Enteroliths are concretions of minerals that cause partial or total obstruction of the intestinal lumen, resulting in recurrent and chronic colic in horses. This pilot study aimed to evaluate the in vitro solvent effect of carbonated beverages (Coca-Cola® and Coca-Cola® Zero), and papain and cellulase enzymes (Robinson Pharma®, Santa Ana, CA, USA) on enteroliths obtained from horses. Six 51-grams samples of six enteroliths were assigned to six treatments of immersion solutions: T1, Coca-Cola®; T2: Coca-Cola® Zero; T3: distilled water + papain (90 mg) and cellulase (120 mg); T4: Coca-Cola® + papain and cellulase; T5: Coca-Cola® Zero + papain and cellulase; and, CT: distilled water (control). The volume for immersion in the assigned solution was 150 mL, at a pH of 7.1, using an incubation shaker (Heidolph® , Germany) at 37ºC and 25 rpm, for 72 h. The evaluation periods of the dissolution percentage (difference between the initial weight and final weight of the samples), were 0, 3, 12, 24, 36, 48, 60, and 72 h. After 72 h of immersion, solutions T4, T5, and T1 presented 47, 38.8, and 14.9% of dissolution, respectively. The other solutions did not have major differences with CT (control). Under the in vitro conditions of this pilot study, papain and cellulase enzymes potentiated the dissolving effect of the carbonated solutions on the enteroliths obtained from horses. Further studies are suggested since the existing literature is on the dissolution of phytobezoars and not of enteroliths. 

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References

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Published

2021-07-12

How to Cite

Vélez Gil, S. A. ., Patiño Marulanda, . J. J. ., & Martínez Aranzales, J. R. (2021). In vitro evaluation of the dissolving effect of carbonated beverages (Coca-Cola® ) and enzyme-based solutions on enteroliths obtained from horses: pilot study. Brazilian Journal of Veterinary Research and Animal Science, 58, e182579. https://doi.org/10.11606/issn.1678-4456.bjvras.2021.182579

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