Use of organic acids to reduce Salmonella Typhimurium excretion in swine

Authors

  • Daniela Gomes da Silva Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Eduarda Alexandra Gonçalves de Oliveira Moura Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Thainara Vitoria Carnevalli Sanches Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Caio Henrique Turco Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Beatriz Belloni Zambotti Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Fernando Antônio Moreira Petri Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Gabriel Yuri Storino Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Henrique Meiroz de Souza Almeida Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Isabela Peixoto Rabelo Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Marina Lopes Mechler-Dreibi Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Karina Sonalio Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias
  • Renato Ravetti Salmix Indústria e Comércio Ltda.
  • Luis Guilherme de Oliveira Universidade Estadual Paulista “Júlio de Mesquita Filho”, Faculdade de Ciências Agrárias e Veterinárias https://orcid.org/0000-0002-1861-5076

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2023.198402

Keywords:

Organic acids, Swine, Salmonella Typhimurium, Euthanasia, Excretion

Abstract

The use of antimicrobials as growth promoters and disease prevention is being constantly reduced in several animal production systems, including in the swine industry. Therefore, this study aimed to evaluate the effectiveness of using acidifiers to control Salmonella Typhimurium in 65-day-old pigs by detecting the pathogen in organs at euthanasia. For this, 24 piglets were divided into two experimental groups consisting of 12 piglets each. An untreated control group (G1) and a treatment group (G2) received a liquid organic acidifier in the drinking water for 10 days (D-5 to D5). Five days after the start of treatment (D0), all piglets were challenged with 106 CFU of Salmonella Typhimurium and assessed for 12 days (D12). Every three days (D3, D6, D9, and D12), three animals from each experimental group were euthanized and then submitted for necropsy. Samples from the intestines (ileum, cecum, mesenteric lymph nodes, and ileocolic lymph nodes), liver, spleen, and lungs were collected to isolate Salmonella. The results show that, numerically, Salmonella isolation in the organs of G2 was lower than in G1 and that the number of positive cecum samples in G1 (66.7%; 8/12) was statistically different from the number of positive models in G2 (16.7%; 2/12), with a reduction of 28.6% of the total cecum positive samples in the treated group compared to the control. Therefore, it was observed that the liquid organic acidifier product could reduce the colonization of organs by Salmonella Typhimurium. 

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References

Ahmed ST, Hwang JA, Hoon J, Mun HS, Yang CJ. Comparison of single and blend acidifiers as alternative to antibiotics on growth performance, fecal microflora, and humoral immunity in weaned piglets. Asian-Australas J Anim Sci. 2014;27(1):93-100. http://dx.doi.org/10.5713/ajas.2013.13411. PMid:25049931.

Argüello H, Carvajal A, Costillas S, Rubio P. Effect of the addition of organic acids in drinking water or feed during part of the finishing period on the prevalence of Salmonella in finishing pigs. Foodborne Pathog Dis. 2013;10(10):842-9. http://dx.doi.org/10.1089/fpd.2013.1497. PMid:23859258.

Berends BR, Van Knapen F, Snijders JMA, Mossel DA. Identification and quantification of risk factors regarding Salmonella spp. on pork carcasses. Int J Food Microbiol. 1997;36(2-3):199-206. http://dx.doi.org/10.1016/S0168-1605(97)01267-1. PMid:9217109.

Berge AC, Wierup M. Nutritional strategies to combat Salmonella in mono-gastric food animal production animal. Animal. 2012;6(4):557-64. http://dx.doi.org/10.1017/S1751731111002217. PMid:22436270.

Bonardi S. Salmonella in the pork production chain and its impact on human health in the european Union. Epidemiol Infect. 2017;145(8):1513-26. http://dx.doi.org/10.1017/S095026881700036X. PMid:28241896.

Borges KM, Oliveira HF, Xavier HPF, Mascarenhas AG. Uso de acidificantes na nutrição de suínos. Nutritime [Internet]. 2015 Abr [cited 2021 Sep 24];12:4004-15. Available from: http://repositorio.bc.ufg.br/handle/ri/18851.

Boyen F, Haesebrouck F, Vanparys A, Volf J, Mahu M, Van Immerseel F, Rychlik I, Dewulf J, Ducatelle R, Pasmans F. Coated fatty acids alter virulence properties of Salmonella Typhimurium and decrease intestinal colonization of pigs. Vet Microbiol. 2008;132(3-4):319-27. http://dx.doi.org/10.1016/j.vetmic.2008.05.008. PMid:18583068.

Braz DB, Costa LB, Berenchtein B, Tse MLP, Almeida VV, Miyada VS. Acidificantes como alternativa aos antimicrobianos promotores do crescimento de leitões. Arch Zootec. 2011;60(231):745-56. http://dx.doi.org/10.4321/S0004-05922011000300062.

Brunelle BW, Bearson SMD, Bearson BL. Salmonella enterica serovar Typhimurium DT104 invasion is not enhanced by sub-inhibitory concentrations of the antibiotic Florfenicol. J Vet Sci Technol. 2011;2:104. http://dx.doi.org/10.4172/2157-7579.1000104.

Busser E. Control of Salmonella in the pork production chain. Ghent: Universiteit Ghent; 2012.

Busser EV, Dewulf J, Nollet N, Houf K, Schwarzer K, Sadeleer L, Zutter L, Maes D. Effect of organic acids in drinking water during the last 2 weeks prior to slaughter on Salmonella shedding by slaughter pigs and contamination of carcasses. Zoonoses Public Health. 2009;56(3):129-36. http://dx.doi.org/10.1111/j.1863-378.2008.01172.x. PMid:18771518.

Busser EV, Dewulf J, Zutter L, Haesebrouck F, Meyns T, Maes W, Maes D. Effect of acidified drinking water at different pH levels on performance and health parameters in nursering pigs. In: International Pig Veterinary Society, organizer. The Proceedings of the 20th Conference of the International Pig Veterinary Society [Internet]; 2008 Jun 22-26; Durban. Durban: Hein Jonker Media Management; c2008. p. 255 [cited 2021 Sep 24]. Available from: http://hdl.handle.net/1854/LU-428226.

Campos J, Mourão J, Peixe L, Antunes P. Non-typhoidal Salmonella in the pig production chain: a comprehensive analysis of its impact on human health. Pathogens. 2019;8(1):19. http://dx.doi.org/10.3390/pathogens8010019. PMid:30700039.

Chattaway MA, Langridge GC, Wain J. Salmonella nomenclature in the genomic era: a time for change. Sci Rep. 2021;11(1):7494. http://dx.doi.org/10.1038/s41598-021-86243-w. PMid:33820940.

Clinical and Laboratory Standards Institute – CLSI. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. VET01S. 3rd ed. Wayne: CLSI; 2020.

Creus E, Pérez JF, Peralta B, Baucells F, Mateu E. Effect of acidified feed on the prevalence of Salmonella in market-age pigs. Zoonoses Public Health. 2007;54(8):314-9. http://dx.doi.org/10.1111/j.1863-2378.2007.01069.x. PMid:17894642.

Denagamage TN, O’Connor AM, Sargeant JM, Rajić A, Mckean JD. Efficacy of vaccination to reduce Salmonella prevalence in live and slaughtered swine: a systematic review of literature from 1979 to 2007. Foodborne Pathog Dis. 2007;4(4):539-49. http://dx.doi.org/10.1089/fpd.2007.0013. PMid:18041963.

Desin TS, Koster W, Potter AA. Salmonella vaccines in poultry: past, present and future. Expert Rev Vaccines. 2013;12(1):87-96. http://dx.doi.org/10.1586/erv.12.138. PMid:23256741.

Fedorka-Cray PJ, Gray JT, Wray C. Salmonella infections in pigs. In: Wray C, Wray A, editors. Salmonella in domestic animals. Wallingford: CABI; 2000. p. 191-207. http://dx.doi.org/10.1079/9780851992617.0191.

Foley SL, Lynne AM, Nayak R. Salmonella challenges: prevalence in swine and poultry and potential pathogenicity of such isolates. J Anim Sci. 2008;86(Suppl 14):E149-62. http://dx.doi.org/10.2527/jas.2007-0464. PMid:17911227.

Freschi CR, Carvalho LFOS, Oliveira CJB. Comparison of DNA-extraction methods and selective enrichment broths on the detection of Salmonella Typhimurium in swine feces by polymerase chain reaction (PCR). Braz J Microbiol. 2005;36(4):363-7. http://dx.doi.org/10.1590/S1517-83822005000400011.

Griffith RW, Carlson SA, Krull AC. Salmonellosis. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW, Zhang J, editors. Diseases of swine. Hoboken: Wiley Blackwell; 2019. p. 912-25. http://dx.doi.org/10.1002/9781119350927.ch59.

Hurd HS, Gailey JK, McKean JD, Rostagno MH. Rapid infection in market-weight swine following exposure to a Salmonella Typhimurium-contaminated environment. Am J Vet Res. 2001;62(8):1194-7. http://dx.doi.org/10.2460/ajvr.2001.62.1194. PMid:11497437.

Hurd HS, McKean JD, Griffith RW, Wesley IV, Rostagno MH. Salmonella enterica infections in market swine with and without transport and holding. Appl Environ Microbiol. 2002;68(5):2376-81. http://dx.doi.org/10.1128/AEM.68.5.2376-2381.2002. PMid:11976111.

Kich JD, Malgarin CM. Controle de Salmonella na suinocultura. In: Associação Brasileira de Veterinários Especialistas em Suínos, organizer. XVII Congresso ABRAVES 2015; 2015 Oct 20-23; Campinas, SP. Porto Alegre (RS): ABRAVES; c2015. p. 98-107.

Michiels J, Missotten J, Rasschaert G, Dierick N, Heyndrickx M, Smet S. Effect of organic acids on Salmonella colonization and shedding in weaned piglets in a seeder model. J Food Prot. 2012;75(11):1974-83. http://dx.doi.org/10.4315/0362-028X.JFP-12-210. PMid:23127706.

Migura L. [Internet]. Barcelona: Slaughterhouse Support Network; c2021 [cited 2021 Sep 24]. Available from: https://sesc.cat/en/the-invisible-zoonosis-i-salmonella-in-pig-meat/.

Oliveira LG, Carvalho LFOS, Masson GCIH, Feliciano MAR. Experimental Infection by Salmonella enterica spp. enterica serovar Panama and tentative of nose-to-nose transmission in weaned pigs. Arq Bras Med Vet Zootec. 2010;62:1340- 7. http://dx.doi.org/10.1590/S0102-09352010000600007.

Ostling CE, Lindgren SE. Inhibition of enterobacteria and Listeria growth by lactic, acetic and formic acids. J Appl Bacteriol. 1993;75(1):18-24. http://dx.doi.org/10.1111/j.1365-2672.1993.tb03402.x. PMid:8365950.

Pesciaroli M, Cucco L, Luca S, Massacci FR, Maresca C, Medici L, Paniccià M, Scoccia E, Staffolani M, Pezzotti G, Magistrali CF. Association between pigs with high caecal Salmonella loads and carcass contamination. Int J Food Microbiol. 2017;242:82-6. http://dx.doi.org/10.1016/j.ijfoodmicro.2016.11.021. PMid:27914322.

Rubin HE, Nerad T, Vaughan F. Lactate acid inhibition of Salmonella typhimurium in yogurt. J Dairy Sci. 1982;65(2):197- 203. http://dx.doi.org/10.3168/jds.S0022-302(82)82177-2. PMid:7042784.

Snary EL, Swart AN, Simons RR, Domingues ARC, Vigre H, Evers EG, Hald T, Hill AA. A quantitative microbiological risk assessment for Salmonella in pigs for the European Union. Risk Anal. 2016;36(3):437-49. http://dx.doi.org/10.1111/risa.12586. PMid:27002672.

Triola MF. Introdução à estatística. 12th ed. Rio de Janeiro: LTC; 2017. 812 p.

Tugnoli B, Giovagnoni G, Piva A, Grilli E. From acidifiers to intestinal health enhancers: how organic acids can improve growth efficiency of pigs. Animals. 2020;10(1):134. http://dx.doi.org/10.3390/ani10010134. PMid:31947627.

Van der Wolf PJ, Bongers JH, Elbers ARW, Franssen FMMC, Hunneman WA, Van Exsel ACA, Tielen MJM. Salmonella infections in finishing pigs in The Netherlands: bacteriological herd prevalence, serogroup and antibiotic resistance of isolates and risk factors for infection. Vet Microbiol. 1999;67(4):263-75. http://dx.doi.org/10.1016/S0378-1135(99)00054-1. PMid:10466502.

Van der Wolf PJ, Van Schie FW, Elbers ARW, Engel B, Van der Heijden HMJF, Hunneman WA, Tielen MJM. Administration of acidified drinking water to finishing pigs in order to prevent Salmonella infections. Vet Q. 2001;23(3):121-5. http://dx.doi.org/10.1080/01652176.2001.9695097. PMid:11513253.

Wood RL, Rose R, Coe NE, Ferris KE. Experimental establishment of persistent infection in swine with a zoonotic strain of Salmonella newport. Am J Vet Res. 1991;52(6):813-9. PMid:1883084.

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Published

2023-03-03

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Vol. 60 (2023)

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FULL ARTICLE

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How to Cite

1.
Silva DG da, Moura EAG de O, Sanches TVC, Turco CH, Zambotti BB, Petri FAM, et al. Use of organic acids to reduce Salmonella Typhimurium excretion in swine. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2023 Mar. 3 [cited 2024 Apr. 26];60:e198402. Available from: https://www.revistas.usp.br/bjvras/article/view/198402