Antagonistic activity of lactic acid bacteria isolated from Minas artisanal cheeses against Brucella abortus

Authors

  • Jamili Maria Suhet Mussi Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Belo Horizonte – MG, Brazil
  • Guilherme Campos Tavares Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Belo Horizonte – MG, Brazil
  • Ethiene Luiza de Souza Santos Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Tecnologia e Inspeção em Produtos de Origem Animal, Belo Horizonte – MG, Brazil
  • Lucas Oliveira Ferreira Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Belo Horizonte – MG, Brazil
  • Damiana Alves de Almeida Rangel Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Tecnologia e Inspeção em Produtos de Origem Animal, Belo Horizonte – MG, Brazil
  • Elaine Maria Seles Dorneles Universidade Federal de Lavras, Faculdade de Zootecnia e Medicina Veterinária, Departamento de Medicina Veterinária, Lavras – MG, Brazil
  • Maysa Serpa Gonçalves Universidade Federal de Lavras, Faculdade de Zootecnia e Medicina Veterinária, Departamento de Medicina Veterinária, Lavras – MG, Brazil
  • Antônio Augusto Fonseca Junior Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Federal de Defesa Agropecuária, Pedro Leopoldo – MG, Brazil
  • Paulo Martins Soares Filho Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Federal de Defesa Agropecuária, Pedro Leopoldo – MG, Brazil
  • Patrícia Gomes de Souza Ministério da Agricultura, Pecuária e Abastecimento, Laboratório Federal de Defesa Agropecuária, Pedro Leopoldo – MG, Brazil
  • Marcelo Resende de Souza Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Tecnologia e Inspeção em Produtos de Origem Animal, Belo Horizonte – MG, Brazil
  • Andrey Pereira Lage Universidade Federal de Minas Gerais, Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Belo Horizonte – MG, Brazil https://orcid.org/0000-0002-9325-5728

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2023.204539

Keywords:

raw milk cheese, milk microbiota, antimicrobial activity, brucellosis, food security

Abstract

This study aimed to evaluate methods for studying the in vitro antimicrobial activity of lactic acid bacteria (LAB) against Brucella abortus and to evaluate the antagonistic effect of LAB on the viability of this pathogen. A total of 18 LAB strains (Lactobacillus plantarum, n = 11; Pediococcus acidilactici, n = 1; Lactobacillus rhamnosus, n = 4; and Lactobacillus brevis, n = 2), isolated from Minas artisanal cheeses produced in three regions (Canastra, Campos das Vertentes, and Araxá) of Minas Gerais State, Brazil, were tested for their antimicrobial activity against B. abortus using three methods: spot-on-lawn, agar well diffusion assay, and antagonistic activity of the culture supernatants. None of the tested LAB strains could inhibit B. abortus in the spot-on-lawn and agar-well diffusion assays. The supernatants produced by LAB had an acidic pH, with intensity depending on bacterial growth and strain, and could inhibit the growth of B. abortus. In contrast, pH-neutralized (pH 7.0) LAB supernatants did not suppress the growth of B. abortus. The results showed that the best technique to study the in vitro antagonism of LAB against B. abortus was the antagonistic activity of culture supernatants. The growth of B. abortus may have been inhibited by acid production.

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References

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2023-08-21

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1.
Mussi JMS, Tavares GC, Santos EL de S, Ferreira LO, Rangel DA de A, Dorneles EMS, et al. Antagonistic activity of lactic acid bacteria isolated from Minas artisanal cheeses against Brucella abortus. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2023 Aug. 21 [cited 2024 Apr. 28];60:e204539. Available from: https://www.revistas.usp.br/bjvras/article/view/204539