Detection mecA gene and Staphylococcus aureus resistance to several antibiotics isolated from cat ear swabs at a veterinary hospital located at Surabaya – Indonesia

Authors

  • Sergius Erikson Kaben Universitas Airlangga, Faculty of Veterinary Medicine, Profession Program in Veterinary Medicine
  • Mohammad Anam Al-Arif Universitas Airlangga, Faculty of Veterinary Medicine, Division of Veterinary Animal Husbandry
  • Anwar Ma’ruf Universitas Airlangga, Faculty of Veterinary Medicine, Division of Basic Veterinary Science
  • Mustofa Helmi Effendi Universitas Airlangga, Faculty of Veterinary Medicine, Division of Veterinary Public Health https://orcid.org/0000-0001-9727-411X
  • Shendy Canadya Kurniawan Wageningen University and Research, Department of Animal Sciences, Master Program of Animal Sciences, Specialisation in Molecule, Cell and Organ Functioning
  • Katty Hendriana Priscilia Riwu Universitas Mandalika, Faculty of Veterinary Medicine, Department of Veterinary Microbiology
  • Daniah Ashri Afnani Universitas Airlangga, Faculty of Veterinary Medicine, Master Program in Veterinary Disease and Public Health
  • Otto Sahat Martua Silaen Universitas Indonesia, Faculty of Medicine, Doctoral Program in Biomedical Science
  • Saumi Kirey Millannia Universitas Airlangga, Faculty of Veterinary Medicine, Profession Program in Veterinary Medicine
  • Safira Ramadhani Universitas Airlangga, Faculty of Veterinary Medicine, Profession Program in Veterinary Medicine
  • Agus Widodo Universitas Airlangga, Faculty of Vocational Studies, Department of Health
  • Thoriq Ihza Farizqi Universitas Airlangga, Faculty of Veterinary Medicine, Profession Program in Veterinary Medicine
  • Aswin Rafif Khairullah Universitas Airlangga, Faculty of Veterinary Medicine, Division of Veterinary Animal Husbandry

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2024.209027

Keywords:

Staphylococcus aureus, Multi-drug resistance, Cat, Public health

Abstract

Cats are susceptible to S. aureus, which mainly colonizes the nose and ears of these feline species. Otitis externa in cat ears is one of the illnesses produced by S. aureus in animals. Antibiotic therapy for affected animals is the conventional treatment for infections by S. aureus. Antibiotic use during prolonged treatment and given at the wrong doses can cause germs to become resistant. Given this context, research on S. aureus isolated from cat ears and tests for antibiotic resistance and the mecA gene is required. Samples of cat ears were obtained from the Amies media using a sterile cotton swab. Bacterial isolation was done on MSA media, and then the catalase and coagulase assays were used to identify the bacteria. S. aureus isolates were evaluated for sensitivity using disks of the antibiotics cefoxitin, tetracycline, erythromycin, gentamicin, and chloramphenicol connected to MHA media. All positive isolates of S. aureus underwent MRSA testing, and then the mecA gene was detected. The sample investigation revealed that 91% (91/100) were positive for S. aureus, and 3.30% (3/91) were confirmed to be multidrug resistant (MDR) because they are resistant to 3–4 antibiotic classes. Out of the 12 MRSA isolates analyzed, the mecA gene was detected in one isolate. Inappropriate antibiotic use causes bacterial resistance in pets. Additionally, excessive antibiotic use in a population might develop acquired bacterial resistance to an antibiotic. Antibiotic use in animals must be assessed to administer medication and prevent the development of antibiotic resistance appropriately.

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2024-04-12

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Kaben SE, Al-Arif MA, Ma’ruf A, Effendi MH, Kurniawan SC, Riwu KHP, et al. Detection mecA gene and Staphylococcus aureus resistance to several antibiotics isolated from cat ear swabs at a veterinary hospital located at Surabaya – Indonesia. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2024 Apr. 12 [cited 2024 Jul. 12];61:e209027. Available from: https://www.revistas.usp.br/bjvras/article/view/209027