Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells

Authors

  • Márcia Dalastra Laurenti Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, Laboratório de Patologia e Doenças Infecciosas, São Paulo, SP
  • Andres Örn Karolinska Institute, Microbiology and Tumorbiology Center, Stockholm
  • Idércio Luiz Sinhorini Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, Laboratório de Patologia e Doenças Infecciosas, São Paulo, SP
  • Carlos Eduardo Pereira Corbett Universidade de São Paulo, Faculdade de Medicina, Departamento de Patologia, Laboratório de Patologia e Doenças Infecciosas, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2005.26440

Keywords:

Natural Killer cells, Cytokines, Cutaneous Leishmaniasis, Leishmania (Leishmania) amazonensis, Immunopathology, 90 Strontium

Abstract

BALBI c mice depleted and non-depleted of Natural Killer (NK) cells were infected subcutaneously with 107 stationary phase promastigotes of Leishmania (Leishmania) amazonensis and samples were taken at 24 hours and 7 days after infection. In NK cell-depleted mice, the NK cytotoxic activity of spleen cells decreased at 7 days after infection and more parasites were found in the lesion. The NK cell populations were analyzed by immunohistochemistry in spleen cryosections. An increase of NK1.1+ expression and a decrease of NK5E6+ antigen expression was observed in NK cell-depleted mice compared to non-depleted mice. When the presence of IFN-g, IL-12 and IL-4 at the site of parasite inoculation was analyzed by immunohistochemistry, a large amount of cytokines was detected in K cell-depleted mice at 24 hours and 7 days after infection. In non-depleted mice, there was a small amount of IL-12 at 24 hours and of IL-4 at 7 days after infection. These data cells suggest that K cell depletion by 90Srresults in increased parasitism in the lesion. The increase of NK1.1+ expression, which mainly produces IL-4, may take part in the progression of the infection.

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Published

2005-04-01

Issue

Section

UNDEFINIED

How to Cite

1.
Laurenti MD, Örn A, Sinhorini IL, Corbett CEP. Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2005 Apr. 1 [cited 2024 Jun. 13];42(2):105-12. Available from: https://www.revistas.usp.br/bjvras/article/view/26440