Density of corneal endothelial cells in eyes of dogs using specular microscopy

Authors

  • João Antonio Tadeu Pigatto Universidade Federal do Rio Grande do Sul, Faculdade de Veterinári, Departamento de Medicina Animal, Porto Alegre, RS
  • Fernando Cesar Abib Universidade Federal do Paraná, Faculdade de Medicina, Departamento de Anatomia, Curitiba, PR
  • Gener Tadeu Pereira Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Ciências Exatas, Jaboticabal, SP
  • Paulo Sergio de Moraes Barros Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Cirurgia, São Paulo, SP
  • Cesar Dias Freire Universidade Federal do Rio Grande do Sul, Faculdade de Veterinária, Porto Alegre, RS
  • José Luiz Laus Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Departamento de Clínica e Cirurgia, Jaboticabal, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26462

Keywords:

Corneal Endothelium, Specular Microscopy, Dog

Abstract

The aim of this study was to examine the endothelial surface and to perform a morphometric analysis of the corneal endothelial cells in normal eyes of dogs using specular microscopy. Morphometric analysis with regard mean cell area and cell density was performed. Both eyes of ten mixed-breed, males and females, with 6 years of age, weighing about 15 kg euthanatized for reasons unrelated to this study were evaluated. Eyes were examined to determine that they did not have visible ocular disease and transported to the laboratory in moist chamber. Using a contact specular microscope the corneal endothelium was examined. Three images of the central corneal endothelium of each eye were obtained. The mean cell area and the cell density of the corneal endothelial cells were obtained using software for corneal endothelium analysis and density measurement. The mean cell area was 395 ± 36 µm² and the endothelial cell density was 2555 ± 240 cells/mm². The present work demonstrates that the normal corneal endothelium of dog is similar to those described in human.

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Published

2006-08-01

Issue

Section

UNDEFINIED

How to Cite

1.
Pigatto JAT, Abib FC, Pereira GT, Barros PS de M, Freire CD, Laus JL. Density of corneal endothelial cells in eyes of dogs using specular microscopy. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2006 Aug. 1 [cited 2024 Apr. 12];43(4):476-80. Available from: https://www.revistas.usp.br/bjvras/article/view/26462