Lignin concentration in oat (Avena byzantina L.) aerial part as measured by four analytical methods

Authors

  • Roseli Sengling Lacerda Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Departamento de Zootecnia, Pirassununga, SP
  • Catarina Abdalla Gomide Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Nutrição e Produção Animal, Pirassununga, SP
  • Romualdo Shigueo Fukushima Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Nutrição e Produção Animal, Pirassununga, SP
  • Renato José Schmidt Universidade de Delaware, Escola de Agricultura e Recursos Naturais, Ciência dos Alimentos e Animal, Delaware, EUA
  • Valdo Rodrigues Herling Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Nutrição e Produção Animal, Pirassununga, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26489

Keywords:

Acetyl bromide, Cell wall, Dioxane, Lignin analyses

Abstract

The current analytical methods to quantify lignin in forages are not satisfactory. A spectrophotometric method, the acetyl bromide lignin (ABL), has been employed to determine lignin concentration in forages; however, it suffers from the lack of an ideal standard with which the optical density readings of samples are compared to. A lignin, extracted from the plant with a solution of acidic dioxane, was employed to build a calibration curve for this method. This procedure was then compared with other methods (acid detergent lignin - ADL, Klason lignin - KL and potassium permanganate lignin - PerL) to determine lignin content on different fractions (stem, leaf and whole plant) of eight oat cultivars (Avena byzantina L.). There was no agreement among the four methods. In general, ABL and KL methods yielded the highest values while ADL method yielded the lowest, particularly for the young plants. Lignin concentration was higher in the stem fraction as compared to leaf. It was detected influence of maturity stage in the investigated samples. It is concluded that the ABL method employing as standard lignin extracted with acidic dioxane has potential to be employed as a method to determine lignin concentration.

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Published

2006-06-01

Issue

Section

UNDEFINIED

How to Cite

1.
Lacerda RS, Gomide CA, Fukushima RS, Schmidt RJ, Herling VR. Lignin concentration in oat (Avena byzantina L.) aerial part as measured by four analytical methods. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2006 Jun. 1 [cited 2024 Jun. 14];43(3):400-7. Available from: https://www.revistas.usp.br/bjvras/article/view/26489