Toxicity test and cryopreservation of sheep isolated preantral follicles using glycerol, ethylen glycol, dimethil sulfoxyde and propanediol

Authors

  • Regiane Rodrigues dos Santos Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais, Fortaleza, CE
  • Ana Paula Ribeiro Rodrigues Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais, Fortaleza, CE
  • Sônia Helena Furtado Costa Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais, Fortaleza, CE
  • Maria Helena Tavares Matos Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais, Fortaleza, CE
  • José Roberto Viana Silva Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais, Fortaleza, CE
  • Juliana Jales de Hollanda Celestino Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais, Fortaleza, CE
  • Fabricio Sousa Martins Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais, Fortaleza, CE
  • Marcia Viviane Alves Saraiva Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais, Fortaleza, CE
  • Mônica Aline Parente Melo Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais, Fortaleza, CE
  • José Ricardo de Figueiredo Universidade Estadual do Ceará, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais, Fortaleza, CE

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26506

Keywords:

Preantral follicles, Sheep, Toxicity test, Cryopreservation

Abstract

The aim of this study was to evaluate isolated sheep preantral follicles (PF) after exposure and cryopreservation using glycerol (GLI), ethylene glycol (EG), propanediol (PROH) or dimethyl sulfoxide (DMSO) at 1.5 and 3.0 M. Each ovarian pair from 5 mixed breed adult sheeps was obtained at a local slaughterhouse and submited to follicular isolation. From the obtained suspension, one aliquot was immediately analysed with trypan blue. The remaining suspension was divided in 16 aliquots of 0.9 mL, suspended in (v/v) in MEM+ with EG, DMSO, GLI or PROH at 1.5 or 3.0 M to the toxicity test and cryopreservation. After the end of each treatment, the follicular viability was analysed and the PF were classified as viable if not dyed or not viable if dyed with trypan blue. The analysis of the results showed that after the toxicity test and cryopreservation, using all cryoprotectants and at both concentrations, the percentage of viable PF was significantly reduced when compared to the control. At the toxicity test, when the cryoprotectants were compared at the same concentrations, the lowest percentage of viable preantral follicles was obtained when 3.0 M PROH (38,9%) was used, being, more toxic when compared to the others cryoprotectants. After cryopreservation, significantly higher percentual of viable PF was observed when the EG and DMSO were used. In conclusion, sheep PF can be cryopreserved successfully using DMSO and EG at 1.5 and 3.0 M.

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Published

2006-04-01

Issue

Section

UNDEFINIED

How to Cite

1.
Santos RR dos, Rodrigues APR, Costa SHF, Matos MHT, Silva JRV, Celestino JJ de H, et al. Toxicity test and cryopreservation of sheep isolated preantral follicles using glycerol, ethylen glycol, dimethil sulfoxyde and propanediol. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2006 Apr. 1 [cited 2024 Jun. 17];43(2):250-5. Available from: https://www.revistas.usp.br/bjvras/article/view/26506