Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses

Authors

  • José Fernando Garcia Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • André Sanches do Amaral Gurgel Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • José Antonio Visintin Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Vagner Ricardo Lunge Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia do Estado do Rio Grande do Sul, Porto Alegre, RS
  • Marcelo Barbante Duarte Rancho’s Tânia, Uberaba, MG
  • Jarbas Leonel Bertolli Associação Brasileira dos Criadores de Cavalo Quarto de Milha, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.2318-3659.v33i3p136-139

Keywords:

Paralysis, Hyperkalemia, Horses, Equidae, DNA.

Abstract

Twelve Quarter horses, descendants from Impressive line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Taq I restriction
enzyme. Accordingly the Hyperkalemic Periodic Paralysis (HYPP) genomic diagnosis was established. The tests revealed that 9 animals were carrier heterozygotes (N/H) and 3 normal homozygotes (N/N). Since the development of PCR methodology, it has been possible to diagnose the problem and plan ways to control the spreading of this defective gene in the population, through detection of carrier and normal animals.

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Published

1996-09-01

Issue

Section

BASIC SCIENCES

How to Cite

1.
Garcia JF, Gurgel AS do A, Visintin JA, Lunge VR, Duarte MB, Bertolli JL. Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses. Braz. J. Vet. Res. Anim. Sci. [Internet]. 1996 Sep. 1 [cited 2024 Apr. 16];33(3):136-9. Available from: https://www.revistas.usp.br/bjvras/article/view/50181