Kidney lesions in goats submitted to intravenous treatment with homologous hemolysate

Abstract

The aim of this study was to evaluate the kidney morphological alterations as consequence of hemoglobinemia. Four male and three female goats were treated with homologous hemolysed blood in an amount of 15 ml/kg/body weight. Blood samples were collected from each animal, submitted to mechanical rupture of red blood cells by freezing process and after from 48 hours collection, were given to the same goat. One of the goat was sacrificed 48 hours after blood sample collection, without receiving any treatment and three other were killed at 3, 9 and 24 hours after being treated with hemolysed blood. The remaining three goats were killed 96 hours after being given the treatment. Kidney fragments were stained by hematoxilin and eosin, alizarine and PAS methods.  Histopathologic alterations were observed as early as 3 hours after the adninistration of hemolysed and remained until 24 hours, when the lesions showed to be progressively more severe. The lesions were
characterized mainly by the presence of proteic droplets, within tubular cells, hemoglobin casts and tubular regenerations (Microvilli destruction). Signs of tubular regenerations were observed in the specimen from the animals killed at 96 hours.