Lymphatic dissemination in neoplasia: determination of nuclear volume and DNA content of primitive and regional lymph node Ehrlich tumor cells

Authors

  • Maria Lúca Zaidan Dagli Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Mitithaka Soma Instituto Butantan, São Paulo, SP
  • José Luiz Guerra Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, São Paulo, SP
  • Paulo Hilário Nascimento Saldiva Universidade de São Paulo, Faculdade de Medicina, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.bjvras.1992.51993

Keywords:

Lymphatic metastasis, Carcinoma Ehrlich tumor, DNA

Abstract

When inoculated into the footpad of mice, Ehrlich tumor grows in a solid form and disseminates to popliteal lymph nodes. This study was performed in order to characterize the tumor cells that migrated from footpad to popliteal lymph nodes. The nuclear volume of primitive and lymph node tumor cells was measured with a caryometric ocular. DNA quantitation was performed in Feulgen stained slides by scanning cytophotometry. Tumor cells harvested from popliteal lymph nodes one hour after inoculation into the footpad showed no statistical differences in DNA quantitation from original ascitic and footpad tumor cells. Tumor cells in popliteal lymph nodes 30 days after inoculation showed a smaller nuclear volume, but presented the same DNA content as the cells that grew in the footpad. Tumor cells in the footpad 30 days after inoculation showed a greater DNA content than those in the footpad one hour after inoculation.These results suggest a possible selective effect for Ehriich tumor cells when they grow in the footpad, but not when they metastasize to regional lymph node.

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Published

1992-12-02

Issue

Section

ANIMAL PATHOLOGY

How to Cite

1.
Dagli MLZ, Soma M, Guerra JL, Saldiva PHN. Lymphatic dissemination in neoplasia: determination of nuclear volume and DNA content of primitive and regional lymph node Ehrlich tumor cells. Braz. J. Vet. Res. Anim. Sci. [Internet]. 1992 Dec. 2 [cited 2024 Apr. 13];29(2):267-71. Available from: https://www.revistas.usp.br/bjvras/article/view/51993