Puncture-biopsy technique for histopatological diagnostic in ovine fascioliasis


  • Paulo Oldemar Scherer Universidade Federal Rural do Rio de Janeiro, Faculdade de Veterinária
  • Edwin Alberto Pile Instituto Oswaldo Cruz, Departamento de Biologia, Rio de Janeiro, RJ
  • Nicolau Maués da Serra-Freire Instituto Oswaldo Cruz, Departamento de Entomologia, Rio de Janeiro, RJ
  • Guido Vidal Schäffer Instituto Oswaldo Cruz, Departamento de Helmintologia, Rio de Janeiro, RJ




Fasciola hepatica, Fascioliasis, Diagnosis, Ovine


In order to evaluate the efficacy of puncture-biopsy technique on the diagnostic of acute, sub-acute or chronic fasciolosis in sheep raised in epidemiological region, an investigation involving two groups of animals was carried out. Group 1 was made up by 65 sheep, which were later slaughterhouse, and Group 2 was made up by 12 animals, which were randomly selected from a single flock from the same area. From the sheep belonging to the Group 1, besides the biopsies, they were sampled faeces and all the livers were evaluated at slaughterhouse. Yet the animals that made up Group 2, besides biopsied, were sampled aiming F. hepatica ova detection under coprologic test. The biopsy’s material was histologically processed and stained by H.E. and Masson methods. All the animals that were positive for F. hepatica under coprologic test, as well as some that were negative, were positives to puncture-biopsy methodology. This technique proved to be an excellent toll for the diagnostic of F. hepatica infection, either acute, sub-acute or chronic. This method was also considered to be more sensitive than faecal single test, mostly in areas which parasite prevalence is known.


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How to Cite

Scherer, P. O., Pile, E. A., Serra-Freire, N. M. da, & Schäffer, G. V. (1999). Puncture-biopsy technique for histopatological diagnostic in ovine fascioliasis. Brazilian Journal of Veterinary Research and Animal Science, 36(4), 219-222. https://doi.org/10.1590/S1413-95961999000400009