Serological survey for bovine herpesvirus 1 in cattle from different regions in the state of Bahia, Brazil
Keywords:Bovine herpesvirus 1 (BHV-1), ELISA, Serum neutralization, Diagnosis.
AbstractThe presence of antibodies against BHV-1 was evaluated in young bovines from fifteen different counties distributed along North, South and Central regions of the state of Bahia. Bovines with age ranged from 1 to 4 years old, non-vaccinated against BHV-1 and without diagnostic of BHV-1 infection were chosen for blood collection. A total of 558 serum samples were collected equivalent to 10% of the population in each farm. The sera were submitted to ELISA (HerdCheck-IDDEX) and Microserum neutralization (MSN) tests. The MSN was performed in monolayers of MDBK cells in microplates using Los Angeles BHV-1 strain. The results of serum samples by ELISA showed that 56% (314/558) were positive and 44% (244/558) were negative. The sera assayed by MSN showed that 48% (269/558) were negative and 52% (289/558) were positive. These positive sera demonstrated that 54% had serumneutralization titer between 4-16, 35% between 32-64 and 11% >; or = 64. Our results suggest that the virus is circulating actively in herds. Furthermore, the high diffusion of the BHV-1 in young bovines could represent a constant source of viral infection in the herd.
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How to Cite
Cerqueira, R. B., Carminati, R., Silva, J. M., Soares, G. C., Meyer, R., & Sardi, S. (2000). Serological survey for bovine herpesvirus 1 in cattle from different regions in the state of Bahia, Brazil. Brazilian Journal of Veterinary Research and Animal Science, 37(6), 497-500. https://doi.org/10.1590/S1413-95962000000600014
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