Comparison of three serological tests applied to diagnosis of the Brucella canis infection in dogs

Authors

  • Sérgio Santos de Azevedo Universidade de São Paulo, Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal da Faculdade, São Paulo, SP
  • Sílvio Arruda Vasconcellos Universidade de São Paulo, Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal da Faculdade, São Paulo, SP
  • Lara Borges Keid Universidade de São Paulo, Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal da Faculdade, São Paulo, SP
  • Lília Márcia Paulin da Silva Grasso Instituto Biológico de São Paulo, São Paulo, SP
  • Sônia Regina Pinheiro Universidade de São Paulo, Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal da Faculdade, São Paulo, SP
  • Roberta Mascolli Universidade de São Paulo, Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal da Faculdade, São Paulo, SP
  • Clebert José Alves Universidade Federal de Campina Grande, Departamento de Medicina Veterinária, Patos, PB

DOI:

https://doi.org/10.1590/S1413-95962004000200005

Keywords:

Serology, Diagnosis, Canine brucellosis, Brucella canis

Abstract

The agar gel immunodiffusion test (AGID), agar gel immunodiffusion test in sera treated by 2-mercaptoethanol (ME-AGID) and complement fixation test (CFT), applied to diagnosis of the canine brucellosis due to Brucella canis, were compared. For this purposes, 80 blood samples from dogs were collected during the rabies vaccination campaign of Santana de Parnaíba municipality, State of São Paulo, in August 1999. The antigens used in the AGID and ME-AGID tests were lipopolysaccharides and proteins from Brucella ovis, strain Reo 198, and in the CFT, the antigen employed was Brucella ovis, strain 63/290. The serological tests were compared two by two by Mc Nemar test, and the agreement was analyzed by the Kappa indicator. The agreement between the CFT and ME-AGID was moderate (Kappa = 0.54), between the AGID and CFT was fair (Kappa = 0.36) and between the AGID and ME-AGID was fair (Kappa = 0.39).

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Published

2004-04-01

Issue

Section

UNDEFINIED

How to Cite

1.
Azevedo SS de, Vasconcellos SA, Keid LB, Grasso LMP da S, Pinheiro SR, Mascolli R, et al. Comparison of three serological tests applied to diagnosis of the Brucella canis infection in dogs. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2004 Apr. 1 [cited 2024 May 28];41(2):106-12. Available from: https://www.revistas.usp.br/bjvras/article/view/6264