Isolation of Leptospira borgpetersenii in synanthropic Didelphis albiventris in Jaboticabal, São Paulo, Brazil

Authors

  • Felipe Jorge da Silva Universidade Estadual Paulista, Jaboticabal, São Paulo
  • Talita Ribeiro Silva Universidade Estadual Paulista, Jaboticabal, São Paulo
  • Glaucenyra Cecília Pinheiro Silva Universidade Estadual Paulista, Jaboticabal, São Paulo
  • Carlos Eduardo Pereira dos Santos Universidade Federal do Mato Grosso, Cuiabá, MT
  • José Roberto Ferreira Alves Júnior Instituto Federal de Goiás, Goiânia, GO
  • Luis Antonio Mathias Universidade Estadual Paulista, Jaboticabal, São Paulo

DOI:

https://doi.org/10.11606/issn.1678-4456.v50i6p457-461

Keywords:

Isolation, Leptospira spp, Brazilian marsupials, MLVA

Abstract

Leptospirosis is a waterborne disease and, therefore, stands out for the possibility of environmental contamination, the cross transmission between domestic and wild animals and humans. Opossum species are important reservoirs of this disease making them potential pathogen spreaders. Aiming to verify the presence of Leptospira spp. and the antibodies against Leptospira spp. in the Campus of São Paulo State University, in Jaboticabal, São Paulo, Brazil, freeliving wild life opossum (Didelphis albiventris) were captured for blood and urine sampling. Serological analysis was performed Microscopic Agglutination Test (MAT). Aliquots of urine were seeded in media Ellinghausen-McCullough- Johnson-Harris (EMJH) and Fletcher without antibiotics. The samples in which there was growth of leptospires were forwarded to the Leptospirosis Laboratory of the Institute of Pathobiology in the National Institute of Agricultural Technology, Buenos Aires, Argentina and were genotyped using Multiple Locus Variable number tandem repeat Analysis (MLVA). Of the 15 analyzed animals, nine (60.0%) were reactant to Patoc serovar. The pathogenic specie Leptospira borgpetersenii was isolated and identified in three Didelphis albiventris. The isolation findings of pathogenic specie Leptopsira borgpetersenii in the urine culture of three Didelphis albiventris in a university campus are a major discovery in the area of preventive veterinary medicine and public health and open a discussion about the important role of free-living wild animals as reservoirs of this agent to domestic animals and humans, a condition that serves as a warning for the improvement of health practices.

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Author Biography

Felipe Jorge da Silva, Universidade Estadual Paulista, Jaboticabal, São Paulo

Graduado em Medicina Veterinária pela Universidade Estadual Paulista, Campus de Jaboticabal; Mestre em Medicina Veterinária, área de Medicina Veterinária Preventiva, pela Universidade Estadual Paulista, Campus de Jaboticabal; Atualmente doutorando do Programa de Pós-graduação em Medicina Veterinária da Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, área de Medicina Veterinária Preventiva.

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Published

2013-12-09

How to Cite

Silva, F. J. da, Silva, T. R., Silva, G. C. P., Santos, C. E. P. dos, Alves Júnior, J. R. F., & Mathias, L. A. (2013). Isolation of Leptospira borgpetersenii in synanthropic Didelphis albiventris in Jaboticabal, São Paulo, Brazil. Brazilian Journal of Veterinary Research and Animal Science, 50(6), 457-461. https://doi.org/10.11606/issn.1678-4456.v50i6p457-461

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