Molecular epidemiology of rabies virus isolated of herbivores from Brazilian Amazon

Authors

  • Haila Chagas Peixoto Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Andrea Isabel Estévez Garcia Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Sheila Oliveira de Souza Silva Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Ofir de Sales Ramos Laboratório Nacional Agropecuário, Belém, PA
  • Lucila Pereira da Silva Laboratório Nacional Agropecuário, Belém, PA
  • Paulo Eduardo Brandão Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP
  • Leonardo José Richtzenhain Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal, São Paulo, SP

DOI:

https://doi.org/10.11606/issn.1678-4456.v51i2p122-130

Keywords:

herbivorous, nucleoprotein, glycoprotein, phylogeny, rabies

Abstract

Rabies virus samples (n = 17) isolated from bovines (n = 11), equines (n = 4) and buffalo (n = 2) from Pará State (n = 7), Tocantins (n = 6) and Rondônia (n = 4) were submitted to RT-PCR in order to obtain partial sequences of nucleoprotein (N) and glycoprotein gene (G). Nucleotide sequences were analyzed using Neighbor-Joining model, Kimura 2-parameters evolutionary model. All the 17 samples analyzed were related to cluster A, lineage associated with the hematophagous bat Desmodus rotundus. The phylogenetic analysis based on the N and G genes, suggests the presence of five sub-lineages (A1-A5), while G gene showed seven sub-lineages (A1-A7). In both phylogenies, sublineages A1 to A3 exhibit a similar composition and geographic distribution. Diverse composition of remaining groups of N and G gene is attributable to different sequences used in the alignments for each genomic region. Glycoprotein amino acid sequence showed molecular markers in sub-lineages A2, A3, A4 and A7. This information provides a better comprehension of molecular epidemiology of rabies, starting with the knowledge of viral lineages circulating in the Brazilian Amazon.

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Published

2014-09-23

Issue

Section

ARTICLES

How to Cite

1.
Peixoto HC, Estévez Garcia AI, Silva SO de S, Ramos O de S, Silva LP da, Brandão PE, et al. Molecular epidemiology of rabies virus isolated of herbivores from Brazilian Amazon. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2014 Sep. 23 [cited 2024 Apr. 12];51(2):122-30. Available from: https://www.revistas.usp.br/bjvras/article/view/64516