Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemper

Authors

  • Adriana Cortez Universidade de Santo Amaro, Medicina Veterinária
  • Vanessa Souza Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia
  • Telma Fernandes Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia
  • Jane Megid Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia

DOI:

https://doi.org/10.11606/issn.1678-4456.v52i4p363-365

Keywords:

Canine distemper, hnRT-PCR, Direct immunofluorescence, Kappa index

Abstract

The diagnostic value of RT-PCR and hemi-nested RT-PCR (hnRT-PCR) was compared in brain samples of dogs presenting neurological signs compatible with canine distemper. Samples of central nervous system (CNS) were collected from 68 dogs and tested by direct immunofluorescence test (RFID) and, independent of the results, they were stored at -20°C for at least three years. They were submitted to the RT-PCR and hnRT-PCR techniques aiming to determine the gene responsible for the viral nucleoprotein decoding. Fifty-nine samples were positive for RIFD, 40 for RT-PCR (Kappa = 0.358) and 54 for hnRT-PCR (Kappa = 0.740). All nine RIFD negative samples were also negative for RT-PCR and hnRT-PCR. In spite of the storage duration and proper sample conditions, the estimated accordance between hnRT-PCR and RIFD demonstrated that hnRT-PCR technique can be applied in retrospective studies.

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Published

2015-12-10

Issue

Section

PRELIMINARY NOTE

How to Cite

1.
Cortez A, Souza V, Fernandes T, Megid J. Evaluation of RT-PCR and hemi-nested RT-PCR in brain samples from dogs with neurologic signs compatible with distemper. Braz. J. Vet. Res. Anim. Sci. [Internet]. 2015 Dec. 10 [cited 2024 Jun. 20];52(4):363-5. Available from: https://www.revistas.usp.br/bjvras/article/view/88333