Morphofunction assessment of ovarian autotransplantation in the retroperitoneum

Abstract

Introduction:

Oophorectomy is required in many pelvic and abdominal procedures. The endocrine disturbances may be followed by severe systemic complications. Objective: In order to maintain the ovarian function after oophorectomy, the present study assessed ovarian autotransplantation in the retroperitoneum. Material and Methods: Thirty-six cycling female Wistar rats were randomly divided into four groups: G1 (control) - sham operation; G2 - bilateral oophorectomy; G3 - bilateral oophorectomy and integre ovarium autotransplantation in the retroperitoneum; G4 - bilateral oophorectomy followed by sliced ovarium autotransplantation in the retroperitoneum. The estrous cycle was investigated in the third and sixth months. Hystologic studies of the ovaries were carried out. Results: The animals of group G1 preserved the cycling sequence. The rats of group G2 remained in diestrus. In group G3, the vaginal smear of two rats showed pattern of estrus, three showed irregular cycles, and the other animals remained in diestrus. In group G4, the rats showed irregular cycles, five presented normal cycle sequence and only one rat remained in diestrous. The histology indicated ovarian viability in Groups 3 and 4, and better aspect in Group 4. Conclusions: Sliced ovarian autotransplantation in the retroperitoneum showed better morphofunction preservation.