Quantitative and qualitative analysis of neuroinflammation by beta amyloid 1-42 toxin after treatment with resveratrol-loaded nanoparticles
DOI:
https://doi.org/10.11606/issn.2176-7262.rmrp.2023.207173Palabras clave:
Nanoparticles, Resveratrol, Alzheimer'sResumen
Background: This study evaluated the effects of zein nanoparticles with resveratrol on neuroinflammation caused by Alzheimer’s disease. Method: The sample consisted of 30 animals divided into control (C), positive control (CP), white nanoparticles (NB), resveratrol nanoparticles (NR) and resveratrol (R) groups. The animals received 10 mg/kg of resveratrol or nanoparticles according to the group, daily, for 15 days, oral administration. Afterward, they were
submitted to immunohistochemical (IHC) analyses. Results: the IHC showed that there was no change in the morphological brain composition in the NR and C groups. Conversely, in the CP, NB, and R groups, changes in the deposition of Anti Tau were observed. The NR group showed a normal projection of taurine in the axon, which was not presented in the same way in the other groups. The CD68 marker showed no microglial activation in the R and C groups. Quantitative analyses of Anti Beta-Amyloid in the NR group showed a statistical difference compared
to the CP, NB, and R groups, whereas the Anti Tau analysis showed a significant difference between the CP and NR groups. The CD68 marker showed a significant difference between the C and NR groups. The analysis of cytokines showed a significant difference in TNF-α between the C and CP groups, C and NB groups, CP and NR groups, and NB and NR groups. IL-6 and InF-δ showed no significant difference between all groups. IL-10 showed significant differences between the C and NR groups, C and R groups, and CP and NR groups. Conclusion: NR prevented the evolution of neuroinflammation.
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Derechos de autor 2023 Mariane Maria Silveira Vieira de Lima, Maria Elvira Ribeiro Cordeiro, Flávio Klinpovous Kerppers, Ketllin Bragnholo, Luiza Ferreira Cunha, Maiara Fonseca, Tatiane Budniak Mazur, Christiane Schineider Machado, Rubiana Mara Mainardes, Afonso Shiguemi Inoue Salgado, André Alexandre Pezzini, Ana Carolina Dorigoni Bini, Ivo Ilvan Kerppers
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
